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Protein Molecular Weight Marker

14-120 kDa Colorimetric
产品编号 ABIN925596
发货至: 中国
  • 检测方法
    Colorimetric
    检测范围
    14-120 kDa
    最低检测浓度
    14 kDa
    应用范围
    SDS-PAGE (SDS), Western Blotting (WB)
    原理
    This protein molecular weight marker is a cocktail containing seven purified proteins that range in size from 14 to 120 kDa and thus span a molecular weight range common to most proteins and protein subunits.
    产品特性
    The protein mixture has been formulated to yield well-defined bands after SDS-polyacrylamide gel electrophoresis and staining with Coomassie blue or other common staining techniques. The intensity of the 67 kDa bovine albumin band has been increased to serve as a reference. The product is supplied in 1X loading buffer and is ready-to-use.
    过滤
    Sterile filtered
  • 应用备注
    This product is designed for use in accurate estimation of protein molecular weight in SDS-polyacrylamide gel electrophoresis and western blotting. Thaw the cocktail at room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 5-10µl is appropriate for most gel formats. These are unstained markers optimized for use with Coomassie blue staining, but the loading volume can be adjusted for compatibility with other staining methods (i.e. silver staining). 62.5 mM Tris-HCl, pH 6.8, 2% (w/v) SDS, 250 mM NaCl, 1mM EDTA, 4% (v/v) β-Mercaptoethanol, 35% (v/v) Glycerol and 0.05% (w/v) bromophenol blue
    说明

    in 1x Loading Buffer

    限制
    仅限研究用
  • 状态
    Liquid
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
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