1Kb DNA Ladder

ABIN1540472 产品详细信息, 供应商: Log in to see
500-10000 bp
500 bp
Agarose Gel Electrophoresis (AGE)
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原理 1Kb DNA ladder (Quantitative) is ideal for determining the size of double-stranded DNA from 500 to 1000 base pairs.
检测方法 Fluorometric
产品特性 The ladder consists of 10 linear double-stranded DNA fragments, chromatography-purified. The intensity of the 2000bp and 5000bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5 µL loading, all fragments except 2000bp and 5000bp are at 40ng. The 2000bp and 5000bp fragments are at 100ng. This ladder is pre-mixed with loading dye and is ready to use.
10 DNA Fragments (bp): 500, 1000, 1500, 2000, 3000, 4000, 5000, 6000, 8000, and 10000
2000bp and 5000 Bands: 100ng/5 µL
Other Bands: 40ng/5 µL
Range: 500bp-10kb
Bands: 10
应用备注 Recommended Loading: 5 µL/Lane.
Add 5 µL ladder directly to each well. You may need more or less than 5 µL of ladder, depending on well size and level of intensity needed to visualize the bands.
Recommended Loading: 5 µL/Lane, 8cm, 1% Agarose Gel, 1×TAE, 7V/cm, 45min.

Do not heat before loading.
For quantification, adjust the concentration of the sample to equalize.
Visualize DNA by staining with ethidium bromide or with SYBR Green I.

限制 仅限研究用
状态 Liquid
储存条件 -20 °C
储存方法 1 year at room temperature, for long term storage, please keep at -20°C.
有效期 12 months
Agarose Gel Electrophoresis (AGE) image for 1Kb DNA Ladder (ABIN1540472) 1Kb DNA Ladder