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|抗原||Paxillin (PXN) 抗体|
|抗原表位||AA 456-472, C-Term 其他选择|
|适用||人, 小鼠, 大鼠 其他选择|
|标记||This Paxillin antibody is un-conjugated 其他选择|
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Paxillin抗体详情Paxillin目标详情 使用细节 贮存及处理 References for anti-Paxillin antibody (ABIN3044187) 图像
|原理||Rabbit IgG polyclonal antibody for Paxillin(PXN) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.|
|交叉反应 （详细）||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Paxillin(PXN) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: paxillin
Protein Name: Paxillin
|纯化方法||Immunogen affinity purified.|
|免疫原||A synthetic peptide corresponding to a sequence at the C-terminal of human Paxillin(456-472aa HEKDGKAYCRKDYFDMF), identical to the related mouse and rat sequences.|
Paxillin目标详情Paxillin抗体详情 使用细节 贮存及处理 References for anti-Paxillin antibody (ABIN3044187) 图像 回页首
|别名||PXN (PXN Antibody 摘要)|
PXN(Paxillin) is a signal transduction adaptor protein discovered in 1990 in the laboratory of Keith Burridge. Salgia et al.(1995) mapped the paxillin gene to 12q24 using fluorescence in situ hybridization.The C-terminal region of paxillin contains four LIM domains that target paxillin to focal adhesions, it is presumed through a direct association with the cytoplasmic tail of beta-integrin. The N-terminal region of paxillin is rich in protein-protein interaction sites. The proteins that bind to paxillin are diverse and include protein tyrosine kinases, such as Src and FAK, structural proteins, such as vinculin and actopaxin, and regulators of actin organization, such as COOL/PIX and PKL/GIT. Paxillin is tyrosine-phosphorylated by FAK and Src upon integrin engagement or growth factor stimulation, creating binding sites for the adapter protein Crk.The paxillin protein contains 4 LIM domains, a proline-rich domain containing a consensus SH3-binding site, and 3 potential SH2-binding sites. On Northern blots, paxillin was expressed as a 3.7-kb mRNA in all tissues tested.
Synonyms: FLJ16691 antibody|FLJ23042 antibody|Paired box protein Pax 1 antibody|PAX 1 antibody|PAX1 antibody|PAXI_HUMAN antibody|Paxillin alpha antibody|Paxillin antibody|PXN antibody|PXN protein antibody
|途径||MAPK Pathway, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling|
使用细节Paxillin抗体详情 Paxillin目标详情 贮存及处理 References for anti-Paxillin antibody (ABIN3044187) 图像 回页首
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
贮存及处理Paxillin抗体详情 Paxillin目标详情 使用细节 References for anti-Paxillin antibody (ABIN3044187) 图像 回页首
|溶解方式||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|缓冲液||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|储存液||Thimerosal (Merthiolate), Sodium azide|
|注意事项||This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.|
|注意事项||Avoid repeated freezing and thawing.|
|储存条件||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
References for anti-Paxillin antibody (ABIN3044187)Paxillin抗体详情 Paxillin目标详情 使用细节 贮存及处理 图像 回页首
Zhu, Liu, Li, Liu, Wang, Sun, Xiong, Jiang, Zheng, Hu: "Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility." in: Carcinogenesis, Vol. 35, Issue 8, pp. 1901-10, 2014
Liu, Zhu, Xiong, Zheng, Hu, Qiu: "Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells." in: International journal of clinical and experimental pathology, Vol. 7, Issue 9, pp. 5750-61, 2014
Zhong, Luo, Wang, Tang, Wu, Wang, Huang, Sun, Huang: "Effects of polydopamine functionalized titanium dioxide nanotubes on endothelial cell and smooth muscle cell." in: Colloids and surfaces. B, Biointerfaces, Vol. 116, pp. 553-60, 2014
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