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|抗原||Mitogen-Activated Protein Kinase 14 (MAPK14) 抗体|
|抗原表位||AA 341-360 其他选择|
|适用||小鸡, 牛, 犬, 豚鼠, Hamster, 人, 猴, 小鼠, 猪, 兔, 大鼠, 绵羊 其他选择|
|标记||This MAPK14 antibody is un-conjugated 其他选择|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
|Supplier||Log in to see|
MAPK14抗体详情MAPK14目标详情 使用细节 贮存及处理 图像
|特异性||Detects ~43 kDa corresponding to the molecular mass of p38 on SDS-PAGE immunoblots.|
|免疫原||A 20 residue synthetic peptide based on the Human p38 (residues 341-360) with the Cysteine residue added and coupled to KLH|
MAPK14目标详情MAPK14抗体详情 使用细节 贮存及处理 图像 回页首
|别名||MAP Kinase p38 alpha / MAPK14 (MAPK14 Antibody 摘要)|
|背景||The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, protein-serine/threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs), extracellular signal-regulated kinases (ERKs), and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).Synonyms: CSBP, CSBP1, CSBP2, CSPB1, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2, MXI2, Mitogen-activated protein kinase 14, SAPK2A, p38 alpha|
|途径||MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling|
使用细节MAPK14抗体详情 MAPK14目标详情 贮存及处理 图像 回页首
Western blot: A 1/5000 dilution of this antibody was sufficient for detection of p38 in 20 μgof HeLa cell lysate by ECL Immunoblot analysis. Immunoprecipitation: 1/250. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
贮存及处理MAPK14抗体详情 MAPK14目标详情 使用细节 图像 回页首
|注意事项||Avoid repeated freezing and thawing.|
|储存条件||4 °C/-20 °C|
|储存方法||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|