anti-人 BAD 抗体 for Immunohistochemistry (Paraffin-embedded Sections)

Recommended BAD Antibody (supplied by: Log in to see )

抗原
BCL2-Associated Agonist of Cell Death (BAD) 抗体
  • bad
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
  • BAD
  • BBC2
  • BCL2L8
  • AI325008
  • Bbc2
  • BCL2-associated agonist of cell death b
  • BCL2 associated agonist of cell death
  • BCL2-associated agonist of cell death
  • badb
  • BAD
  • Bad
抗原表位
AA 140-168
59
59
48
47
47
45
45
39
32
22
19
19
19
19
19
19
19
19
19
19
19
19
19
19
18
18
18
18
18
18
15
14
14
11
10
10
10
9
9
8
8
7
6
5
5
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
适用
人, 小鼠
786
471
301
19
19
8
5
4
3
2
2
1
1
1
1
1
1
宿主

1001
47
2
1
克隆类型
多克隆
标记
This BAD antibody is un-conjugated
67
66
66
60
60
60
6
6
6
6
6
6
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
应用范围
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
554
540
418
352
129
78
54
33
14
13
10
7
4
3
2
1
1
1
选项
Supplier
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Supplier Product No.
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Available images

产品编号 ABIN674709
$ 306.90
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.389673 ABIN461986 IHC IHC (p) IP WB Mouse IgG N-Term Log in to see BYC001 0
12.389673 ABIN4282857 IHC IHC (p) WB Rabbit Log in to see Polyclonal 1
12.389673 ABIN213744 IHC IHC (p) WB Rabbit IgG Ser112 Log in to see Polyclonal 0
12.389673 ABIN214418 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
12.389673 ABIN498774 IHC (p) WB Rabbit pSer112 Log in to see Polyclonal 0
12.389673 ABIN498190 IF IHC (p) WB Rabbit Log in to see Polyclonal 0
12.389673 ABIN498864 IHC (p) Rabbit Log in to see Polyclonal 0
12.389673 ABIN682828 IF (p) IHC (p) WB Rabbit IgG pSer75 Log in to see Polyclonal 0
12.389673 ABIN756562 IF (p) IHC (p) WB Rabbit IgG pSer118 Log in to see Polyclonal 0
12.389673 ABIN498091 IHC (p) WB Rabbit Log in to see Polyclonal 0
12.389673 ABIN4282865 IHC (p) IP WB Mouse IgG N-Term Log in to see BYC001 0
12.389673 ABIN4282859 ICC IF IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 0
12.389673 ABIN5557534 IHC (p) WB Rabbit IgG Log in to see 5D4 0
12.389673 ABIN4282868 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
12.389673 ABIN4282853 ICC IF IHC IHC (p) WB Rabbit pSer155 Log in to see Polyclonal 0
12.389673 ABIN5949401 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG Log in to see SA32-01 0
12.389673 ABIN4282864 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 0
1 ABIN358087 EIA IHC (p) WB Rabbit Ig Fraction pSer99 Log in to see Polyclonal 5
1 ABIN389519 IHC (p) WB Rabbit Ig Fraction pSer134 Log in to see Polyclonal 2
1 ABIN388118 FACS IHC (p) WB Rabbit Ig Fraction AA 92-127, BH3 Domain Log in to see Polyclonal 3

General

抗原 BCL2-Associated Agonist of Cell Death (BAD) 抗体
抗原表位 AA 140-168
(59), (59), (48), (47), (47), (45), (45), (39), (32), (22), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (18), (18), (18), (18), (18), (18), (15), (14), (14), (11), (10), (10), (10), (9), (9), (8), (8), (7), (6), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用 人, 小鼠
(786), (471), (301), (19), (19), (8), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
宿主
(1001), (47), (2), (1)
克隆类型
多克隆
标记 This BAD antibody is un-conjugated
(67), (66), (66), (60), (60), (60), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
应用范围 Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(554), (540), (418), (352), (129), (78), (54), (33), (14), (13), (10), (7), (4), (3), (2), (1), (1), (1)
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BAD抗体详情

BAD目标详情 使用细节 贮存及处理 图像
纯化方法 Purified by Protein A.
免疫原 KLH conjugated synthetic peptide derived from human Bad
亚型 IgG
Plasmids, Primers & others

BAD目标详情

BAD抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 Bad (BAD Antibody 摘要)
背景

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Subcellular location: Cytoplasm, Mitochondrion

Synonyms: BBC2, BCL2L8, Bcl2-associated agonist of cell death, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, BBC6

基因ID 572
UniProt Q92934
途径 MAPK Pathway, PI3K-Akt Signaling, RTK signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events

使用细节

BAD抗体详情 BAD目标详情 贮存及处理 图像 回页首
应用备注 WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
限制 仅限研究用

贮存及处理

BAD抗体详情 BAD目标详情 使用细节 图像 回页首
状态 Liquid
浓度 1 μg/μL
缓冲液 Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store at -20°C
有效期 12 months

图像

BAD抗体详情 BAD目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Western Blotting (WB) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Lane 1:Mouse kidney lysate Lane 2: Mouse brain lysate probed with Rabbit Anti-Bad Pol...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Bad Polyclonal A...
Western Blotting (WB) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Image provided by the Independent Validation Program (badge number 29802). Lane 1: MC...
'独立验证'标志
Lot Number 090915
Method validated Western Blotting
Positive Control MCF7 cells
Negative Control C6/36 cells (non-reactive species)
Notes A strong band was observed in the positive control sample at the correct molecular weight. No bands were observed in the negative control sample.
Primary Antibody
  • Antigen: BCL2-Associated Agonist of Cell Death (BAD)
  • Catalog number: ABIN674709
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 090915
  • Antibody Dilution: 1:200
Secondary Antibody
  • Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: L170-6515
  • Antibody Dilution: 1:10,000
Controls
  • Positive control: MCF7 cells
  • Negative control: C6/36 cells
实验流程
  • 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
  • 2. 15 μl of heated extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 16 hours at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 minutes each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
  • 11. The membrane was rinsed in TBS twice for 30 seconds each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 600 seconds.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
  • 15. The membrane was washed in TBST 2 times for 10 minutes each.
  • 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
Experimental Notes - No experimental challenges noted.
Validation Images
Western Blotting validation image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Figure 1: Western Blot for BAD. Arrowhead indicates the expected molecular weight of ...