MANF 293 Cell Lysate

ABIN1595759 产品详细信息, 供应商: Log in to see
  • ARP
  • 3230402M22Rik
  • AA407711
  • AA408789
  • AA673178
  • Armet
  • D17914
  • D18Mgi17
  • MANF
  • RBM15B
  • ARP-like
  • CG7013
  • DmMANF
  • Dmel\\CG7013
  • anon-EST:ParkEST188
  • arp
  • armet
  • im:6909481
  • zgc:153327
  • manf
  • mesencephalic astrocyte derived neurotrophic factor
  • mesencephalic astrocyte-derived neurotrophic factor
  • RNA binding motif protein 15B
  • Mesencephalic astrocyte-derived neurotrophic factor
  • mesencephalic astrocyte-derived neurotrophic factor S homeolog
  • MANF
  • Manf
  • RBM15B
  • manf
  • manf.S

ELISA, Immunoprecipitation (IP), Western Blotting (WB)
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Supplier Product No.
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品牌 TACL™
产品特性 Antigen standard for mesencephalic astrocyte-derived neurotrophic factor (MANF) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC221555, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
组件 This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer.
Lysate Fraction Whole Cell Lysate
Lysate Type Cell Lysate
Lysed Cells HEK 293T Cells
背景 Alternate Names: arginine-rich protein, arginine-rich, mutated in early stage tumors, MANF, ARMET, ARP, MGC142148, MGC142150, Mesencephalic astrocyte-derived neurotrophic factor, arginine-rich mutated in early stage tumor
NCBI登录号 NP_006001
应用备注 WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 μg lysate per lane.
限制 仅限研究用
状态 Liquid
缓冲液 Each lysate vial contains 0.1 mg lysate in 0.1 mL (1 mg/mL) of RIPA Buffer (50 mM Tris-HCl pH 7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 % NP40). The loading buffer vial contains 0.5 mL 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10 % glycerol, 4 % SDS, 0.002 % Bromophenol blue, 5 % beta-mercaptoethanol).
注意事项 Minimize freeze-thaw cycles.
储存条件 -20 °C,-80 °C
储存方法 Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
有效期 6 months
Western Blotting (WB) image for MANF 293 Cell Lysate (ABIN1595759) MANF lysate validation: The MANF 293 Cell Lysate (lane B) and Control Vector Cell Lys...