EDN1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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- 抗原 See all Endothelin 1 (EDN1) products
- Endothelin 1 (EDN1)
- 宿主
- 人
- 资源
- 人细胞
- 应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- 原理
- Transient Overexpression Lysate
- 特异性
- Non-denatured
- 产品特性
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EDN1 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-EDN1 full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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- 应用备注
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- 说明
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Product Quality tested by: Transient overexpression cell lysate was tested with Anti-EDN1 antibody by Western Blots.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2 mg/mL
- 缓冲液
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- 注意事项
- Aliquot to avoid repeated freezing and thawing.
- 储存条件
- -80 °C
- 储存方法
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
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- 抗原
- Endothelin 1 (EDN1)
- 别名
- EDN1 (EDN1 产品)
- 别名
- et1 Lysate, et-1 Lysate, Edn1 Lysate, EDN Lysate, ET-1 Lysate, ET1 Lysate, PPET1 Lysate, HDLCQ7 Lysate, preproET Lysate, Et1 Lysate, fb14d01 Lysate, wu:fb14d01 Lysate, PPET-1 Lysate, EDN1 Lysate, edn1 Lysate, edn1-a Lysate, edn1-b Lysate, endothelin 1 Lysate, endothelin 1 S homeolog Lysate, endothelin 1 L homeolog Lysate, edn1 Lysate, EDN1 Lysate, Edn1 Lysate, edn1.S Lysate, edn1.L Lysate
- 背景
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Full Gene Name: endothelin 1
Synonyms: ET1,HDLCQ7 - 基因ID
- 1906
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