CYB5 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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- 抗原 See all CYB5A products
- CYB5A (Cytochrome B5 Type A (Microsomal) (CYB5A))
- 宿主
- 人
- 资源
- 人细胞
- 应用范围
- Immunoprecipitation (IP), Western Blotting (WB)
- 原理
- Transient Overexpression Lysate
- 特异性
- Non-denatured
- 产品特性
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CYB5 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-CYB5 full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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- 应用备注
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- 说明
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Product Quality tested by: Transient overexpression cell lysate was tested with Anti-CYB5 antibody by Western Blots.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2 mg/mL
- 缓冲液
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- 注意事项
- Aliquot to avoid repeated freezing and thawing.
- 储存条件
- -80 °C
- 储存方法
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
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- 抗原
- CYB5A (Cytochrome B5 Type A (Microsomal) (CYB5A))
- 别名
- CYB5A (CYB5A 产品)
- 别名
- CYB5 Lysate, MCB5 Lysate, Cyb5 Lysate, cyb5 Lysate, zgc:64123 Lysate, wu:fb44a11 Lysate, MGC80327 Lysate, 0610009N12Rik Lysate, Cyb5a Lysate, CYB5A Lysate, cytochrome b5 type A Lysate, cytochrome b5 type A (microsomal) Lysate, cytochrome b5 type A (microsomal) L homeolog Lysate, cytochrome b5 Lysate, CYB5A Lysate, Cyb5a Lysate, cyb5a Lysate, cyb5a.L Lysate, LOC100328915 Lysate, LOC100735904 Lysate
- 背景
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Full Gene Name: cytochrome b5 type A (microsomal)
Synonyms: CYB5,MCB5 - 基因ID
- 1528
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