bmMSCs(human bone marrow-derived mesenchymal stem cell) whole cell lysate (non-denatured)
(1 reference)-
- 宿主
- 人
- 资源
- 人细胞
- 应用范围
- Immunoprecipitation (IP), Western Blotting (WB)
- 原理
- Whole cell lysate
- 特异性
- Non-denatured
- 产品特性
-
b mMSCs(human bone marrow-derived mesenchymal stem cell) whole cell lysate (non-denatured)
Used Tissue: Bone Marrow - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Cell Lysate
- Lysate Fraction
- Whole Cell Lysate
-
CYP26A1 293T Cell Transient Overexpression Lysate(Denatured)
CYP26A1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
BIRC3 293T Cell Transient Overexpression Lysate(Denatured)BIRC3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
APLP2 293T Cell Transient Overexpression Lysate(Denatured)APLP2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
BAG1 293T Cell Transient Overexpression Lysate(Denatured)BAG1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
VNN1 293T Cell Transient Overexpression Lysate(Denatured)VNN1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
THP-1 (human acute monocytic leukemia) whole cell lysate (non-denatured)IP, WB Whole Cell Lysate Cell Lysate THP-1 Cells 适用: 人
Rat liver tissue lysate (non-denatured)IP, WB Whole Tissue Lysate Tissue Lysate 适用: 大鼠
Rat brain tissue lysate (non-denatured)IP, WB Whole Tissue Lysate Tissue Lysate 适用: 大鼠
SHISA5 293T Cell Transient Overexpression Lysate(Denatured)SHISA5 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
S100P 293T Cell Transient Overexpression Lysate(Denatured)S100P WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
-
- 应用备注
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- 说明
-
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- 注意事项
- Aliquot to avoid repeated freezing and thawing.
- 储存条件
- -80 °C
- 储存方法
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
-
-
: "Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 3, pp. 679-85, (2006) (PubMed).
-
: "Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 3, pp. 679-85, (2006) (PubMed).
-
- 背景
-
Full Gene Name:
Synonyms:
-
