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TR-FRET Assays

TR-FRET (Time-Resolved Fluorescence Energy Transfer) is a special form of FRET (Förster Resonance Energy Transfer) in which the long-lived emission fluorophores are used as donors. Sandwich TR-FRET immunoassays for semi-quantitative detection enable the examination of phosphorylation events in cellular signaling pathways. We offer high quality TR-FRET Assay Kit alternatives at an outstanding price.

Unlike other TR-FRET kits, we include a positive control in every kit to allow results you can depend on.
Our Phospho-Protein Kits enable the detection of proteins phosphorylated at defined residues.
Total-Protein Kits allow the detection of total protein in a sample and can be used to normalize your data obtained with the Phospho-Kits.
The Phospho- and Total Protein Kits are designed for simultaneous measurement of two proteins (phosphorylated and total) in separate wells from the same cell lysate using a single kit.

Our kits come in convenient sizes of 100 and 500 points for 69 key targets.

Highly validated components and Data-transparency

Comprehensive Technical Data Sheets showing the pharmacological validation of each assay kit are available. Browse our available TR-FRET assays down below! Our support team is happy to help you select the proper kit for your research. We can answer any questions you may have. Please contact us via phone, email or use our live chat system.

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74 results:

Phospho-4EBP1 (T37/T46) and Total 4EBP1 TR-FRET Cellular Assay Kit Kit

Synonym: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 CA, TR-FRET 适用: 人, 小鼠, 大鼠, 猴 Fluorometric Cell Lysate Semi-Quantitative

A431 cells were grown in three T-175 flasks at 37°C, 5%CO2, in DMEM with 10% FBS and cultured to 70% confluence.

A431 cells were grown in three T-175 flasks at 37°C, 5% CO2, in DMEM with 10% FBS and cultured to 70% confluence.

A431 cells were plated at 25,000 cells per well in 96-well microplate.

HEK293 cells were plated at different cell densities in a 96-well microplate.

4 images

产品编号 ABIN6938945

$1,196.84

加上运输费 $45.00

500 points

发货至: 美国

2至4个工作日

Phospho-CREB (S133) and Total CREB TR-FRET Cellular Assay Kit Kit

Synonym: cAMP Responsive Element Binding Protein 1 CA, TR-FRET 适用: 人, 小鼠, 大鼠 Fluorometric Cell Lysate Semi-Quantitative

8x10^7 of B lymphocyte cells grown in RPMI with 15% FBS were stimulated with 20 nM of NECA (8.

A431 cells were plated at 50,000 cells per well in 96-well microplate.

80 million (8.

NIH 3T3 cells were plated at 50,000 cells per well in 96-well microplate.

4 images

产品编号 ABIN6938956

$1,196.84

加上运输费 $45.00

500 points

发货至: 美国

2至4个工作日

Phospho-STAT6 (Y641) and Total STAT6 TR-FRET Cellular Assay Kit Kit

CA, TR-FRET 适用: 人, 小鼠, 大鼠 Fluorometric Cell Lysate Semi-Quantitative

HeLa cells were seeded at 40,000 cells/well and cultured overnight at 37 °C, 5 % CO2, in DMEM with 10 % FBS.

HeLa cells were grown in a T175 flask at 37 °C, 5 % CO2, in DMEM with 10 % FBS and cultured to 60 % confluence.

HeLa cells were grown in a T175 flask at 37 °C, 5 % CO2, in DMEM with 10 % FBS and cultured to 60 % confluence.

HeLa cells were seeded at 40,000 cells/well and cultured overnight at 37 °C, 5 % CO2, in DMEM with 10 % FBS.

4 images

产品编号 ABIN6938969

$1,196.84

加上运输费 $45.00

500 points

发货至: 美国

2至4个工作日

Phospho-Ribosomal Protein S6 (S240/244) and Total Ribosomal Protein S6 TR-FRET Cellular Assay Kit Kit

CA, TR-FRET 适用: 人, 小鼠, 大鼠 Fluorometric Cell Lysate Semi-Quantitative

MCF-7 cells were grown in a T175 flask at 37 °C, 5 % CO2, in EMEM with 10 % FBS, 10 % non-essential amino-acids and cultured to 90 % confluence.

MCF-7 cells were grown in a T175 flask at 37 °C, 5 % CO2, in EMEM with 10 % FBS, 10 % non-essential amino-acids and cultured to 90 % confluence.

MCF-7 cells were seeded at 75,000 cells/well and cultured overnight at 37 °C, 5 % CO2, in EMEM with 10 % FBS and 10 % non-essential amino-acids.

MCF-7 cells were seeded at 75,000 cells/well and cultured overnight at 37 °C, 5 % CO2, in EMEM with 10 % FBS and 10 % non-essential amino-acids.

4 images

产品编号 ABIN6938973

$1,196.84

加上运输费 $45.00

500 points

发货至: 美国

2至4个工作日

Phospho-Ribosomal Protein S6 (S235/236) and Total Ribosomal Protein S6 TR-FRET Cellular Assay Kit Kit

CA, TR-FRET 适用: 人, 小鼠, 大鼠 Fluorometric Cell Lysate Semi-Quantitative

MCF-7 cells were seeded at 75,000 cells/well and cultured overnight at 37 °C, 5 % CO2, in EMEM with 10 % FBS and 10 % non-essential amino-acids.

MCF-7 cells were grown in a T175 flask at 37 °C, 5 % CO2, in EMEM with 10 % FBS, 10 % non-essential amino-acids and cultured to 90 % confluence.

MCF-7 cells were grown in a T175 flask at 37 °C, 5 % CO2, in EMEM with 10 % FBS, 10 % non-essential amino-acids and cultured to 90 % confluence.

MCF-7 cells were seeded at 75,000 cells/well and cultured overnight at 37 °C, 5 % CO2, in EMEM with 10 % FBS and 10 % non-essential amino-acids.

4 images

产品编号 ABIN6938975

$1,196.84

加上运输费 $45.00

500 points

发货至: 美国

2至4个工作日

Phospho-STAT5 (Y694/Y699) and Total STAT5 TR-FRET Cellular Assay Kit Kit

CA, TR-FRET 适用: 人, 小鼠, 大鼠 Fluorometric Cell Lysate Semi-Quantitative

A431 cells were seeded at 75,000 cells/well and cultured overnight at 37 °C, 5 % CO2, in DMEM with 10 % FBS.

A431 cells were grown in a T175 flask at 37 °C, 5 % CO2, in DMEM with 10 % FBS and cultured to 95 % confluence.

A431 cells were grown in a T175 flask at 37 °C, 5 % CO2, in DMEM with 10 % FBS and cultured to 95 % confluence.

A431 cells were seeded at 75,000 cells/well and cultured overnight at 37 °C, 5 % CO2, in DMEM with 10 % FBS.

4 images

产品编号 ABIN6938990

$1,196.84

加上运输费 $45.00

500 points

发货至: 美国

2至4个工作日

Phospho-Met (Y1234/1235) and Total MET TR-FRET Cellular Assay Kit Kit

CA, TR-FRET 适用: 人 Fluorometric Cell Lysate Semi-Quantitative

HeLa cells were grown in a T175 flask at 37°C, 5% CO2 in DMEM with 10% FBS and cultured to 90% confluence.

HeLa cells were grown in a T175 flask at 37°C, 5% CO2 in DMEM with 10% FBS and cultured to 90% confluence.

HeLa (50,000 cells/well) were treated with increasing concentrations of HGF for 10 min at room temperature before lysis with 50 µL of Lysis Buffer 5 containing 1 mM NaF, 2 mM Na3VO4, Roche Minicomplete (without EDTA) protease inhibitor cocktail, 0.

HeLa cells were plated at 50,000 cells per well in 96-well microplate.

4 images

产品编号 ABIN6939009

$1,196.84

加上运输费 $45.00

500 points

发货至: 美国

2至4个工作日

Phospho-4EBP1 (T37/T46) TR-FRET Cellular Assay Kit Kit

Synonym: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 CA, TR-FRET 适用: 人, 小鼠, 大鼠, 猴 Fluorometric Cell Lysate Semi-Quantitative

A431 cells were plated at 25,000 cells per well in 96-well microplate.

HEK293 cells were plated at different cell densities in a 96-well microplate.

A431 cells were grown in three T-175 flasks at 37°C, 5% CO2, in DMEM with 10% FBS and cultured to 70% confluence.

3 images

产品编号 ABIN6938946

$353.25

加上运输费 $45.00

100 points (More quantities available)

发货至: 美国

2至4个工作日

Phospho-CREB (S133) TR-FRET Cellular Assay Kit Kit

Synonym: cAMP Responsive Element Binding Protein 1 CA, TR-FRET 适用: 人, 小鼠, 大鼠 Fluorometric Cell Lysate Semi-Quantitative

A431 cells were plated at 50,000 cells per well in 96-well microplate.

NIH 3T3 cells were plated at 50,000 cells per well in 96-well microplate.

80 million (8.

3 images

产品编号 ABIN6938957

$353.25

加上运输费 $45.00

100 points (More quantities available)

发货至: 美国

2至4个工作日

Phospho-Ribosomal Protein S6 (S240/244) TR-FRET Cellular Assay Kit Kit

CA, TR-FRET 适用: 人, 小鼠, 大鼠 Fluorometric Cell Lysate Semi-Quantitative

MCF-7 cells were seeded at 75,000 cells/well and cultured overnight at 37 °C, 5 % CO2, in EMEM with 10 % FBS and 10 % non-essential amino-acids.

MCF-7 cells were grown in a T175 flask at 37 °C, 5 % CO2, in EMEM with 10 % FBS, 10 % non-essential amino-acids and cultured to 90 % confluence.

MCF-7 cells were seeded at 75,000 cells/well and cultured overnight at 37 °C, 5 % CO2, in EMEM with 10 % FBS and 10 % non-essential amino-acids.

3 images

产品编号 ABIN6938974

$353.25

加上运输费 $45.00

100 points (More quantities available)

发货至: 美国

2至4个工作日

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