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TR-FRET Assays

TR-FRET (Time-Resolved Fluorescence Energy Transfer) is a special form of FRET (Förster Resonance Energy Transfer) in which the long-lived emission fluorophores are used as donors.

For the investigation of protein interactions, methods have been established which exploit the energy transfer from fluorescence light to a second fluorophore (FRET). This only takes place when two molecules are in close proximity, for example when two proteins interact. This requires two proteins that are coupled to different fluorophores: a donor that emits light after excitation and only excites the acceptor fluorophore when the bound proteins interact. The ratio of acceptor to donor fluorescence provides information on the association of both proteins.

Time Resolved FRET Cellular Kinase Assay

The TR-FRET Cellular Kinase Assay Kits are intended for the simple and rapid detection of either phosphorylated or total protein in cell lysates using the homogeneous (no-wash) TR-FRET technology. Cells are first treated to modulate protein levels. Cells are then lysed with the specific lysis buffer provided in the kit and the target protein is subsequently detected in one-step using the TR-FRET reagents. The target protein (phosphorylated or total) is detected in a sandwich assay format using 2 different labelled specific antibodies. The transmitter of the fluorescence emission is preferably made up of molecules with a long emission lifetime (time resolved FRET, TR-FRET). This allows the signal to be detected at a later point in time and over a longer period of time and minimizes background fluorescence. Excitation of the donor with a flash lamp (320 or 340 nm) or a laser (337 nm) triggers a Fluorescence Resonance Energy Transfer (FRET) towards the acceptor, which in turn emits light at 665 nm. The signal at 665 nm is proportional to the concentration of protein in the cell lysate.

Browse our available TR-FRET assays down below! Our support team will gladly help you with finding the right kit for your needs or answer occuring questions. Please contact us via phone, email or use our live chat system.

68 results:

Phospho-Met (Y1234/1235) and Total MET TR-FRET Cellular Assay Kit Kit

CA, TR-FRET 适用: 人 Fluorometric Semi-Quantitative Cell Lysate
产品编号 ABIN6939009
$1,476.00
加上运输费 $45.00
500 points
local_shipping 发货至: 美国
2至4个工作日

Phospho-4EBP1 (T37/T46) and Total 4EBP1 TR-FRET Cellular Assay Kit Kit

Synonym: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 CA, TR-FRET 适用: 人, 猴, 小鼠, 大鼠 Fluorometric Semi-Quantitative Cell Lysate
产品编号 ABIN6938945
$1,476.00
加上运输费 $45.00
500 points
local_shipping 发货至: 美国
2至4个工作日

Phospho-CREB (S133) and Total CREB TR-FRET Cellular Assay Kit Kit

Synonym: cAMP Responsive Element Binding Protein 1 CA, TR-FRET 适用: 人, 小鼠, 大鼠 Fluorometric Semi-Quantitative Cell Lysate
产品编号 ABIN6938956
$1,476.00
加上运输费 $45.00
500 points
local_shipping 发货至: 美国
2至4个工作日

Phospho-BAD (S112) TR-FRET Cellular Assay Kit Kit

CA, TR-FRET 适用: 人, 猴, 小鼠, 大鼠 Fluorometric Semi-Quantitative Cell Lysate
产品编号 ABIN6938981
$436.00
加上运输费 $45.00
100 points (More quantities available)
local_shipping 发货至: 美国
2至4个工作日

Phospho-Met (Y1234/1235) TR-FRET Cellular Assay Kit Kit

CA, TR-FRET 适用: 人 Fluorometric Semi-Quantitative Cell Lysate
产品编号 ABIN6939010
$436.00
加上运输费 $45.00
100 points (More quantities available)
local_shipping 发货至: 美国
2至4个工作日

Phospho-4EBP1 (T37/T46) TR-FRET Cellular Assay Kit Kit

Synonym: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 CA, TR-FRET 适用: 人, 猴, 小鼠, 大鼠 Fluorometric Semi-Quantitative Cell Lysate
产品编号 ABIN6938946
$436.00
加上运输费 $45.00
100 points (More quantities available)
local_shipping 发货至: 美国
2至4个工作日

Phospho-CREB (S133) TR-FRET Cellular Assay Kit Kit

Synonym: cAMP Responsive Element Binding Protein 1 CA, TR-FRET 适用: 人, 小鼠, 大鼠 Fluorometric Semi-Quantitative Cell Lysate
产品编号 ABIN6938957
$436.00
加上运输费 $45.00
100 points (More quantities available)
local_shipping 发货至: 美国
2至4个工作日

Total Met TR-FRET Cellular Assay Kit Kit

CA, TR-FRET 适用: 人 Fluorometric Semi-Quantitative Cell Lysate
产品编号 ABIN6939011
$436.00
加上运输费 $45.00
100 points (More quantities available)
local_shipping 发货至: 美国
2至4个工作日

Total 4EBP1 TR-FRET Cellular Assay Kit Kit

Synonym: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 CA, TR-FRET 适用: 人, 猴, 小鼠, 大鼠 Fluorometric Semi-Quantitative Cell Lysate
产品编号 ABIN6938947
$436.00
加上运输费 $45.00
100 points (More quantities available)
local_shipping 发货至: 美国
2至4个工作日

Total CREB TR-FRET Cellular Assay Kit Kit

Synonym: cAMP Responsive Element Binding Protein 1 CA, TR-FRET 适用: 人, 小鼠, 大鼠 Fluorometric Semi-Quantitative Cell Lysate
产品编号 ABIN6938958
$436.00
加上运输费 $45.00
100 points (More quantities available)
local_shipping 发货至: 美国
2至4个工作日

Phospho-SMAD2 (S465/S467) TR-FRET Cellular Assay Kit Kit

CA, TR-FRET 适用: 人, 小鼠, 大鼠 Fluorometric Semi-Quantitative Cell Lysate
产品编号 ABIN6938968
$436.00
加上运输费 $45.00
100 points (More quantities available)
local_shipping 发货至: 美国
2至4个工作日

Phospho-STAT6 (Y641) TR-FRET Cellular Assay Kit Kit

CA, TR-FRET 适用: 人, 小鼠, 大鼠 Fluorometric Semi-Quantitative Cell Lysate
产品编号 ABIN6938970
$436.00
加上运输费 $45.00
100 points (More quantities available)
local_shipping 发货至: 美国
2至4个工作日

Total STAT6 TR-FRET Cellular Assay Kit Kit

CA, TR-FRET 适用: 人, 小鼠, 大鼠 Fluorometric Semi-Quantitative Cell Lysate
产品编号 ABIN6938971
$436.00
加上运输费 $45.00
100 points (More quantities available)
local_shipping 发货至: 美国
2至4个工作日

Phospho-Ribosomal Protein S6 (S235/236) TR-FRET Cellular Assay Kit Kit

CA, TR-FRET 适用: 人, 小鼠, 大鼠 Fluorometric Semi-Quantitative Cell Lysate
产品编号 ABIN6938972
$436.00
加上运输费 $45.00
100 points (More quantities available)
local_shipping 发货至: 美国
2至4个工作日

Phospho-Ribosomal Protein S6 (S240/244) TR-FRET Cellular Assay Kit Kit

CA, TR-FRET 适用: 人, 小鼠, 大鼠 Fluorometric Semi-Quantitative Cell Lysate
产品编号 ABIN6938974
$436.00
加上运输费 $45.00
100 points (More quantities available)
local_shipping 发货至: 美国
2至4个工作日
  • 类型 Kit
    • Kit
  • 抗原
    • Ribosomal Protein S6 (RPS6)
    • V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
    • Epidermal Growth Factor Receptor (EGFR)
    • Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A)
    • Eukaryotic Translation Initiation Factor 4E (EIF4E)
    • Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1)
    • Glycogen Synthase Kinase 3 beta (GSK3b)
    • Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2)
    • Met Proto-Oncogene (MET)
    • Mitogen-Activated Protein Kinase 1/3 (MAPK1/3)
    • Mitogen-Activated Protein Kinase 8 (MAPK8)
    • Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)
    • Nuclear Factor kappa B (NFkB)
    • Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1)
    • STAT5 A/B
    • Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3)
    • Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6)
    • Tumor Protein P53 (TP53)
    • cAMP Responsive Element Binding Protein 1 (CREB1)
    • BCL2-Associated Agonist of Cell Death (BAD)
    • Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
    • Insulin Receptor beta (ISR-beta)
    • Mitogen-Activated Protein Kinase 14 (MAPK14)
    • Proto-oncogene tyrosine-protein kinase Src (Src)
    • SMAD, Mothers Against DPP Homolog 2 (SMAD2)
    • p38 alpha beta gamma
  • 应用范围 Time-resolved Fluorescence Energy Transfer (TR-FRET)
    • Time-resolved Fluorescence Energy Transfer (TR-FRET)
    • ELISA
    • Biochemical Assay (BCA)
    • Functional Studies (Func)
    • Activity Assay (AcA)
    • Detection (D)
    • Quantification (Q)
    • Western Blotting (WB)
    • Enzyme Activity Assay (EAA)
    • Cellular Assay (CA)
    • Labeling (Lbl)
    • Screening Assay (ScA)
    • Flow Cytometry (FACS)
    • ELISpot
    • Conjugation (Con)
    • Chromatin Immunoprecipitation (ChIP)
    • Fluorescence Resonance Energy Transfer Microscopy (FRET)
    • Purification (Purif)
    • Binding Studies (Bind)
    • Immunoprecipitation (IP)
    • Immunofluorescence (IF)
    • Immunohistochemistry (IHC)
    • Apoptosis Detection (AD)
    • Mass Spectrometry (MS)
    • Inhibition Assay (InhA)
    • Colloidal Gold Immunochromatography Assay (GICA)
    • Immunocytochemistry (ICC)
    • Protein Complex Immunoprecipitation (Co-IP)
    • Immunohistochemistry (Frozen Sections) (IHC (fro))
    • Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    • High Pressure Liquid Chromatography (HPLC)
    • Pull-Down Assay (Pull-Down)
    • Affinity Measurement (AM)
    • Fluorescence Microscopy (FM)
    • Activation (Act)
    • Immunoassay (IA)
    • Cell Viability Assay (CVA)
    • High-throughput Screening (HTS)
    • Rapid Test (rapid)
    • Cytotoxicity Test (CyTox)
    • Proliferation Assay (ProA)
    • Isotyping (IsoT)
    • Staining Methods (StM)
    • Separation (Sep)
    • Cell Culture (CC)
    • ChIP DNA-Sequencing (ChIP-seq)
    • Light Microscopy (LM)
    • Sample Preparation (Prep)
    • ChIP quantitative PCR (ChIP-qPCR)
    • Protein Extraction (PEx)
      Please use search to find more...
  • 适用
    • 小鼠
    • 大鼠
    • Cow
    • Hamster
    • Pig
    • Mink
    • 斑马鱼
  • 图像
  • Analytical Method
    • Semi-Quantitative
  • 检测方法
    • Fluorometric
  • 抗原表位
    • total
    • pSer133
    • pSer15
    • pSer209
    • pSer218
    • pSer222
    • pSer235
    • pSer236
    • pSer240
    • pSer244
    • pSer376
    • pSer473
    • pSer51
    • pSer536
    • pSer9
    • pThr180
    • pThr183
    • pThr202
    • pThr308
    • pThr37
    • pThr389
    • pThr46
    • pTyr1068
    • pTyr1234
    • pTyr1235
    • pTyr182
    • pTyr185
    • pTyr204
    • pTyr641
    • pTyr694
    • pTyr705
    • pSer112
    • pSer465
    • pSer467
    • pTyr1150
    • pTyr1151
    • pTyr419
  • 样本
    • Cell Lysate