电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

IKKi/IKKe ELISA 试剂盒

IKBKE 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6951456
发货至: 中国
  • 抗原 See all IKKi/IKKe (IKBKE) ELISA试剂盒
    IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))
    适用
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human IKK-E/IKBKE ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling IKBKE ELISA Kit
    Top Product
    Discover our top product IKBKE ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all IKKi/IKKe (IKBKE) ELISA试剂盒
    IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))
    别名
    IKK-E (IKBKE (IKBKE 产品)
    别名
    zgc:100844 ELISA Kit, IKBKE ELISA Kit, MGC130907 ELISA Kit, IKK-E ELISA Kit, IKK-i ELISA Kit, IKKE ELISA Kit, IKKI ELISA Kit, AW558201 ELISA Kit, IKKepsilon ELISA Kit, Ikki ELISA Kit, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon ELISA Kit, inhibitor of nuclear factor kappa B kinase subunit epsilon ELISA Kit, inhibitor of nuclear factor kappa B kinase subunit epsilon L homeolog ELISA Kit, inhibitor of kappaB kinase epsilon ELISA Kit, IKBKE ELISA Kit, ikbke ELISA Kit, ikbke.L ELISA Kit, Ikbke ELISA Kit
    基因ID
    9641
    UniProt
    Q14164
    途径
    TLR signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
You are here:
客服