IKBKB ELISA 试剂盒
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- 抗原 See all IKBKB ELISA试剂盒
- IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human IKK-B ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product IKBKB ELISA Kit
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Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) ELISA Kit
IKBKB 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) ELISA KitIKBKB 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) ELISA KitIKBKB 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) ELISA KitIKBKB 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all IKBKB ELISA试剂盒
- IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))
- 别名
- IKK-B (IKBKB 产品)
- 别名
- ikk-beta ELISA Kit, ikk2 ELISA Kit, ikkb ELISA Kit, ikkbeta ELISA Kit, IKBKB ELISA Kit, IKKB ELISA Kit, CG4201 ELISA Kit, DIK ELISA Kit, DLAK ELISA Kit, DmIKK ELISA Kit, DmIKKBeta ELISA Kit, DmIKKb ELISA Kit, DmIKKbeta ELISA Kit, DmIkkbeta ELISA Kit, Dmel\\CG4201 ELISA Kit, Dmikkb ELISA Kit, Dmikkbeta ELISA Kit, IK ELISA Kit, IKK ELISA Kit, IKK-beta ELISA Kit, IKKb ELISA Kit, IKKbeta ELISA Kit, IRD-5 ELISA Kit, IRD5 ELISA Kit, Ik ELISA Kit, Ikkb ELISA Kit, Ikkbeta ELISA Kit, Ird-5 ELISA Kit, Ird5 ELISA Kit, LRK ELISA Kit, Lak ELISA Kit, anon-89Bd ELISA Kit, dIKK ELISA Kit, dIKK-beta ELISA Kit, dIKKbeta ELISA Kit, dLak ELISA Kit, dmIKKbeta ELISA Kit, ikk ELISA Kit, ird ELISA Kit, ird5/DmIkk[beta] ELISA Kit, IKK2 ELISA Kit, NFKBIKB ELISA Kit, AIM-1 ELISA Kit, AI132552 ELISA Kit, IKK-2 ELISA Kit, IKK[b] ELISA Kit, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta L homeolog ELISA Kit, inhibitor of nuclear factor kappa B kinase subunit beta ELISA Kit, I-kappaB kinase beta ELISA Kit, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta ELISA Kit, inhibitor of kappaB kinase beta ELISA Kit, ikbkb.L ELISA Kit, IKBKB ELISA Kit, ikbkb ELISA Kit, IKKbeta ELISA Kit, Ikbkb ELISA Kit
- 基因ID
- 3551
- UniProt
- O14920
- 途径
- NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins
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