AGT ELISA 试剂盒
-
- 抗原 See all AGT ELISA试剂盒
- AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))
-
适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse AGT ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse AGT.
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product AGT ELISA Kit
-
Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) ELISA Kit
AGT 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Cell Culture Supernatant, Plasma, Serum
Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) ELISA KitAGT 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) ELISA KitAGT 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) ELISA KitAGT 适用: 大鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) ELISA KitAGT 适用: Various Species Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) ELISA KitAGT 适用: 人 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) ELISA KitAGT 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) ELISA KitAGT 适用: 小鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) ELISA KitAGT 适用: 犬 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all AGT ELISA试剂盒
- AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))
- 别名
- AGT (AGT 产品)
- 别名
- ANHU ELISA Kit, SERPINA8 ELISA Kit, AI265500 ELISA Kit, AngI ELISA Kit, AngII ELISA Kit, Aogen ELISA Kit, Serpina8 ELISA Kit, ANRT ELISA Kit, Ang ELISA Kit, PAT ELISA Kit, wu:fb62f06 ELISA Kit, wu:fj87b02 ELISA Kit, zgc:111892 ELISA Kit, AGT ELISA Kit, angt ELISA Kit, ANGT ELISA Kit, angiotensinogen ELISA Kit, angiotensinogen (serpin peptidase inhibitor, clade A, member 8) ELISA Kit, AGT ELISA Kit, Agt ELISA Kit, agt ELISA Kit
- UniProt
- P11859
- 途径
- JAK/STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Monocarboxylic Acid Catabolic Process, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Regulation of long-term Neuronal Synaptic Plasticity
-
