PRKAR1A ELISA 试剂盒
PRKAR1A
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all PRKAR1A products
- PRKAR1A (Protein Kinase, CAMP-Dependent, Regulatory, Type I, alpha (Tissue Specific Extinguisher 1) (PRKAR1A))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human PRKAR1A ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human PRKAR1A.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all PRKAR1A products
- PRKAR1A (Protein Kinase, CAMP-Dependent, Regulatory, Type I, alpha (Tissue Specific Extinguisher 1) (PRKAR1A))
- 别名
- PRKAR1A (PRKAR1A 产品)
- 别名
- ACRDYS1 ELISA Kit, ADOHR ELISA Kit, CAR ELISA Kit, CNC ELISA Kit, CNC1 ELISA Kit, PKR1 ELISA Kit, PPNAD1 ELISA Kit, PRKAR1 ELISA Kit, TSE1 ELISA Kit, 1300018C22Rik ELISA Kit, RIalpha ELISA Kit, Tse-1 ELISA Kit, Tse1 ELISA Kit, RIIA ELISA Kit, prkar1a ELISA Kit, zgc:92515 ELISA Kit, im:7047729 ELISA Kit, cnc1 ELISA Kit, pkr1 ELISA Kit, ppnad1 ELISA Kit, prkar1 ELISA Kit, tse1 ELISA Kit, protein kinase cAMP-dependent type I regulatory subunit alpha ELISA Kit, protein kinase, cAMP dependent regulatory, type I, alpha ELISA Kit, protein kinase cAMP-dependent type 1 regulatory subunit alpha ELISA Kit, protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a ELISA Kit, cAMP-dependent protein kinase type I-alpha regulatory subunit pseudogene ELISA Kit, protein kinase cAMP-dependent type I regulatory subunit alpha S homeolog ELISA Kit, PRKAR1A ELISA Kit, Prkar1a ELISA Kit, prkar1aa ELISA Kit, LOC707275 ELISA Kit, prkar1a.S ELISA Kit
- UniProt
- P10644
- 途径
- Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma
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