Activin A Receptor Type IB/ALK-4 ELISA 试剂盒
ACVR1B
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all Activin A Receptor Type IB/ALK-4 (ACVR1B) ELISA试剂盒
- Activin A Receptor Type IB/ALK-4 (ACVR1B) (Activin A Receptor, Type IB (ACVR1B))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse Activin RIB/ALK-4 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse Activin RIB.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product ACVR1B ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all Activin A Receptor Type IB/ALK-4 (ACVR1B) ELISA试剂盒
- Activin A Receptor Type IB/ALK-4 (ACVR1B) (Activin A Receptor, Type IB (ACVR1B))
- 别名
- Activin RIB (ALK-4 (ACVR1B 产品)
- 别名
- ACVR1B ELISA Kit, alk4 ELISA Kit, skr2 ELISA Kit, alk-4 ELISA Kit, xalk4 ELISA Kit, actrib ELISA Kit, acvrlk4 ELISA Kit, DKFZp469A1614 ELISA Kit, 6820432J04 ELISA Kit, ActR-IB ELISA Kit, ActRIB ELISA Kit, Acvrlk4 ELISA Kit, Alk4 ELISA Kit, SKR2 ELISA Kit, ACTRIB ELISA Kit, ACVRLK4 ELISA Kit, ALK4 ELISA Kit, Skr2 ELISA Kit, axis ELISA Kit, taram-a ELISA Kit, tarama ELISA Kit, wu:fb75f01 ELISA Kit, activin A receptor type 1B ELISA Kit, Activin receptor type-1B ELISA Kit, activin A receptor, type IB ELISA Kit, activin A receptor, type 1B ELISA Kit, activin A receptor type 1Ba ELISA Kit, ACVR1B ELISA Kit, acvr1b ELISA Kit, acv1b ELISA Kit, Acvr1b ELISA Kit, acvr1ba ELISA Kit
- UniProt
- Q61271
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