TWSG1 ELISA 试剂盒
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- 抗原 See all TWSG1 ELISA试剂盒
- TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))
- 适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse TSG ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse TSG.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Twisted Gastrulation Homolog 1 (TWSG1) ELISA Kit
TWSG1 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Twisted Gastrulation Homolog 1 (TWSG1) ELISA KitTWSG1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all TWSG1 ELISA试剂盒
- TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))
- 别名
- TSG (TWSG1 产品)
- 别名
- TWSG1 ELISA Kit, TSG ELISA Kit, DKFZp469J051 ELISA Kit, 1810013J15Rik ELISA Kit, 9030422N06Rik ELISA Kit, AW552143 ELISA Kit, D17Ertd403e ELISA Kit, Tsg ELISA Kit, Twg ELISA Kit, fi23f01 ELISA Kit, tsg ELISA Kit, tsga ELISA Kit, twsg1 ELISA Kit, wu:fi23f01 ELISA Kit, twsg ELISA Kit, tsg1 ELISA Kit, tsgb ELISA Kit, ztsg1 ELISA Kit, twisted gastrulation BMP signaling modulator 1 ELISA Kit, twisted gastrulation homolog 1 (Drosophila) ELISA Kit, twisted gastrulation BMP signaling modulator 1a ELISA Kit, twisted gastrulation BMP signaling modulator 1 S homeolog ELISA Kit, twisted gastrulation BMP signaling modulator 1 L homeolog ELISA Kit, twisted gastrulation BMP signaling modulator 1b ELISA Kit, TWSG1 ELISA Kit, Twsg1 ELISA Kit, twsg1a ELISA Kit, twsg1.S ELISA Kit, twsg1.L ELISA Kit, twsg1b ELISA Kit
- UniProt
- Q9EP52
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