Prosaposin ELISA 试剂盒
PSAP
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all Prosaposin (PSAP) ELISA试剂盒
- Prosaposin (PSAP)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse PSAP ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse PSAP.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product PSAP ELISA Kit
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Prosaposin (PSAP) ELISA Kit
PSAP 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Seminal Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Prosaposin (PSAP) ELISA试剂盒
- Prosaposin (PSAP)
- 别名
- PSAP (PSAP 产品)
- 别名
- GLBA ELISA Kit, SAP1 ELISA Kit, AI037048 ELISA Kit, SGP-1 ELISA Kit, SGP1A ELISA Kit, cb759 ELISA Kit, wu:fa14a06 ELISA Kit, wu:fb36e02 ELISA Kit, wu:fb58g07 ELISA Kit, PSAP ELISA Kit, PSP-A ELISA Kit, SFTPA1B ELISA Kit, SP-A ELISA Kit, prosaposin ELISA Kit, surfactant protein A1 ELISA Kit, PSAP ELISA Kit, Psap ELISA Kit, psap ELISA Kit, LOC100011417 ELISA Kit, LOC100304726 ELISA Kit, LOC100526719 ELISA Kit, SFTPA1 ELISA Kit
- UniProt
- Q61207
- 途径
- Positive Regulation of Endopeptidase Activity
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