S100A8 ELISA 试剂盒
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- 抗原 See all S100A8 ELISA试剂盒
- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.65 ng/mL - 150 ng/mL
- 最低检测浓度
- 0.65 ng/mL
- 应用范围
- ELISA
- 原理
- Mouse S100A8/S100A9 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse S100A8.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product S100A8 ELISA Kit
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S100 Calcium Binding Protein A8 (S100A8) ELISA Kit
S100A8 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 适用: Cow Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 适用: 人 AA 2-93 Colorimetric Sandwich ELISA
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 适用: 小鼠 AA 2-89 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 试剂准备
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Recommended Dilution for serum and plasma samples2 fold
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all S100A8 ELISA试剂盒
- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
- 别名
- S100A8 (S100A9 (S100A8 产品)
- 别名
- 60B8AG ELISA Kit, CAGA ELISA Kit, CFAG ELISA Kit, CGLA ELISA Kit, CP-10 ELISA Kit, L1Ag ELISA Kit, MA387 ELISA Kit, MIF ELISA Kit, MRP8 ELISA Kit, NIF ELISA Kit, P8 ELISA Kit, Mrp8 ELISA Kit, S100A8 ELISA Kit, 60B8Ag ELISA Kit, AI323541 ELISA Kit, B8Ag ELISA Kit, CFAg ELISA Kit, Caga ELISA Kit, p8 ELISA Kit, MRP-8 ELISA Kit, S100 calcium binding protein A8 ELISA Kit, S100 calcium binding protein A8 (calgranulin A) ELISA Kit, S100A8 ELISA Kit, S100a8 ELISA Kit
- 途径
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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