TNFRSF10B ELISA 试剂盒
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- 抗原 See all TNFRSF10B ELISA试剂盒
- TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse TRAIL R2/TNFRSF10B ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse TRAIL R2.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all TNFRSF10B ELISA试剂盒
- TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))
- 别名
- TRAIL R2 (TNFRSF10B (TNFRSF10B 产品)
- 别名
- TNFRSF10B ELISA Kit, dr5 ELISA Kit, cd262 ELISA Kit, killer ELISA Kit, trick2 ELISA Kit, trickb ELISA Kit, xDR-M1 ELISA Kit, xDR-M2 ELISA Kit, ztnfr9 ELISA Kit, trailr2 ELISA Kit, trick2a ELISA Kit, trick2b ELISA Kit, CD262 ELISA Kit, DR5 ELISA Kit, KILLER ELISA Kit, KILLER/DR5 ELISA Kit, TRAIL-R2 ELISA Kit, TRAILR2 ELISA Kit, TRICK2 ELISA Kit, TRICK2A ELISA Kit, TRICK2B ELISA Kit, TRICKB ELISA Kit, ZTNFR9 ELISA Kit, Ly98 ELISA Kit, MK ELISA Kit, tumor necrosis factor receptor superfamily, member 10b ELISA Kit, tumor necrosis factor receptor superfamily member 10b S homeolog ELISA Kit, tumor necrosis factor receptor superfamily member 10b L homeolog ELISA Kit, tumor necrosis factor receptor superfamily member 10b ELISA Kit, TNF receptor superfamily member 10b ELISA Kit, Tnfrsf10b ELISA Kit, TNFRSF10B ELISA Kit, tnfrsf10b.S ELISA Kit, tnfrsf10b.L ELISA Kit, tnfrsf10b ELISA Kit
- UniProt
- Q9QZM4
- 途径
- p53 Pathway, Apoptosis, Positive Regulation of Endopeptidase Activity
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