HGF ELISA 试剂盒
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- 抗原 See all HGF ELISA试剂盒
- HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 125 pg/mL - 8000 pg/mL
- 最低检测浓度
- 125 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - citrate), Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: DFNB39, F TCF, Fibroblast derived tumor cytotoxic factor, Hepapoietin A, Hepatocyte growth factor, Hepatocyte growth factor (hepapoietin A scatter factor, Hepatocyte growth factor beta chain, Hepatopoietin-A, Hgf, HGF_HUMAN, HGFB, HPTA, Lung fibroblast derived mitogen, Scatter factor, SF
Background: Hepatocyte growth factor(HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass(Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.
Gene Name: HGF
Production: Natural and recombinant human HGF. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: sf21, Immunogen sequence: Q32-R494(alpha)&V495-S728(beta)
- Top Product
- Discover our top product HGF ELISA Kit
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL,4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human HGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(EDTA, citrate) to each empty well. It is recommended that each human HGF standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Sodium EDTA
ELISA Dilution: 125pg/mL-8000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- 抗原 See all HGF ELISA试剂盒
- HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
- 别名
- HGF (HGF 产品)
- 别名
- DFNB39 ELISA Kit, F-TCF ELISA Kit, HGFB ELISA Kit, HPTA ELISA Kit, SF ELISA Kit, HGF ELISA Kit, C230052L06Rik ELISA Kit, HGF/SF ELISA Kit, NK1 ELISA Kit, NK2 ELISA Kit, SF/HGF ELISA Kit, hepatocyte growth factor ELISA Kit, HGF ELISA Kit, Hgf ELISA Kit
- 基因ID
- 3082
- NCBI登录号
- NP_000592
- UniProt
- P14210
- 途径
- RTK signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
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