CDKN1B IQ-ELISA 试剂盒
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- 抗原 See all CDKN1B IQ-ELISA Kits
- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
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适用
- 大鼠
- 检测方法
- qPCR
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Rat Immunoquantitative (PCR-Based) p27/Kip1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Semi-Quantitative
- 产品特性
- IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
- 组件
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- p27/Kip1 Microplate (Item A): 96 well PCR plate coated with anti-Rat p27/Kip1
- Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
- Standards (Item C): 2 vials of recombinant Rat p27/Kip1
- Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
- Detection Affinity Reagent for p27/Kip1 (Item F): 2 vials of a 4x concentrated solution of anti-Rat p27/Kip1 affinity reagent
- IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
- Primer Solution (Item I): 1.7 ml vial
- PCR Master Mix (Item J): 1.2 ml vial
- PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
- Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
- 试剂未包括
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- Real-time PCR instrument, Bio-Rad recommended
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for data analysis
- Tubes to prepare standard or sample dilutions
- Heating block or water bath capable of 80°C
- Featured
- Discover our best selling CDKN1B ELISA Kit
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- Discover our top product CDKN1B ELISA Kit
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Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) ELISA Kit
CDKN1B 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) ELISA KitCDKN1B 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) ELISA KitCDKN1B 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
- 说明
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The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.
- 样本量
- 25 µL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed
- Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
- Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
- Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
- Add 15μL Primer solution 10μL of PCR master mix to each well
- Run real-time PCR
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
- 有效期
- 6 months
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- 抗原 See all CDKN1B IQ-ELISA Kits
- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
- 别名
- p27/Kip1 (CDKN1B 产品)
- 别名
- CDKN4 IQ-ELISA Kit, KIP1 IQ-ELISA Kit, MEN1B IQ-ELISA Kit, MEN4 IQ-ELISA Kit, P27KIP1 IQ-ELISA Kit, cdkn1b IQ-ELISA Kit, sb:cb611 IQ-ELISA Kit, wu:fb63g10 IQ-ELISA Kit, wu:fb64g10 IQ-ELISA Kit, wu:fe18e03 IQ-ELISA Kit, CDKN1B IQ-ELISA Kit, Kip1 IQ-ELISA Kit, p27 IQ-ELISA Kit, FAM14D IQ-ELISA Kit, ISG12 IQ-ELISA Kit, ISG12A IQ-ELISA Kit, P27 IQ-ELISA Kit, Cdki1b IQ-ELISA Kit, AA408329 IQ-ELISA Kit, AI843786 IQ-ELISA Kit, p27Kip1 IQ-ELISA Kit, cdkn1bl IQ-ELISA Kit, cyclin dependent kinase inhibitor 1B IQ-ELISA Kit, cyclin-dependent kinase inhibitor 1Bb IQ-ELISA Kit, interferon alpha inducible protein 27 IQ-ELISA Kit, cyclin-dependent kinase inhibitor 1B IQ-ELISA Kit, cyclin-dependent kinase inhibitor 1Ba IQ-ELISA Kit, CDKN1B IQ-ELISA Kit, cdkn1bb IQ-ELISA Kit, IFI27 IQ-ELISA Kit, Cdkn1b IQ-ELISA Kit, cdkn1ba IQ-ELISA Kit
- 基因ID
- 83571
- UniProt
- O08769
- 途径
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
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