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TNFRSF1A ELISA 试剂盒

TNFRSF1A 适用: 小鼠 Colorimetric Sandwich ELISA 1-300 pg/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN625426
发货至: 中国
  • 抗原 See all TNFRSF1A ELISA试剂盒
    TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    1-300 pg/mL
    最低检测浓度
    1 pg/mL
    应用范围
    ELISA
    原理
    Mouse TNF RI (TNFRSF1A) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair detects mouse sTNFRI. Other species not determined.
    灵敏度
    1 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Recommended Dilution for serum and plasma samples5 - 50 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • Vergori, Lauret, Gaceb, Beauvillain, Andriantsitohaina, Martinez: "PPAR? regulates endothelial progenitor cell maturation and myeloid lineage differentiation through a NADPH oxidase-dependent mechanism in mice." in: Stem cells (Dayton, Ohio), Vol. 33, Issue 4, pp. 1292-303, (2015) (PubMed).

  • 抗原 See all TNFRSF1A ELISA试剂盒
    TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
    别名
    TNF RI (TNFRSF1A 产品)
    别名
    CD120a ELISA Kit, FPF ELISA Kit, MS5 ELISA Kit, TBP1 ELISA Kit, TNF-R ELISA Kit, TNF-R-I ELISA Kit, TNF-R55 ELISA Kit, TNFAR ELISA Kit, TNFR1 ELISA Kit, TNFR1-d2 ELISA Kit, TNFR55 ELISA Kit, TNFR60 ELISA Kit, p55 ELISA Kit, p55-R ELISA Kit, p60 ELISA Kit, TNF-R1 ELISA Kit, TNF-alphaR1 ELISA Kit, TNFRI ELISA Kit, TNFRp55 ELISA Kit, TNFalpha-R1 ELISA Kit, Tnfr-2 ELISA Kit, Tnfr1 ELISA Kit, fpf ELISA Kit, tbp1 ELISA Kit, p55-r ELISA Kit, tnf-r ELISA Kit, tnfar ELISA Kit, tnfr1 ELISA Kit, cd120a ELISA Kit, tnfr55 ELISA Kit, tnfr60 ELISA Kit, xtnfr1 ELISA Kit, wu:fj65b10 ELISA Kit, zgc:85914 ELISA Kit, TNFR I ELISA Kit, TNFRSF1A ELISA Kit, TNFA ELISA Kit, cTNF ELISA Kit, TNF receptor superfamily member 1A ELISA Kit, tumor necrosis factor receptor superfamily, member 1a ELISA Kit, TNF receptor superfamily member 1A S homeolog ELISA Kit, tumor necrosis factor ELISA Kit, TNFRSF1A ELISA Kit, Tnfrsf1a ELISA Kit, tnfrsf1a.S ELISA Kit, tnfrsf1a ELISA Kit, TNF ELISA Kit
    背景
    Gene Names: Tnfrsf1a Tnfr-1 Tnfr1
    Protein names: Tumor necrosis factor receptor superfamily member 1A (Tumor necrosis factor receptor 1) (TNF-R1) (Tumor necrosis factor receptor type I) (TNF-RI) (TNFR-I) (p55) (p60) (CD antigen CD120a)
    基因ID
    21937
    UniProt
    P25118
    途径
    NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway
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