TNFRSF21 ELISA 试剂盒
TNFRSF21
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
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- 抗原 See all TNFRSF21 ELISA试剂盒
- TNFRSF21 (Tumor Necrosis Factor Receptor Superfamily, Member 21 (TNFRSF21))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Death Receptor 6 (DR6) ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes human DR6.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all TNFRSF21 ELISA试剂盒
- TNFRSF21 (Tumor Necrosis Factor Receptor Superfamily, Member 21 (TNFRSF21))
- 别名
- DR6 (TNFRSF21 产品)
- 别名
- TNFRSF21 ELISA Kit, tnfrsf21 ELISA Kit, MGC146356 ELISA Kit, BM-018 ELISA Kit, CD358 ELISA Kit, DR6 ELISA Kit, AA959878 ELISA Kit, R74815 ELISA Kit, TR7 ELISA Kit, dr6 ELISA Kit, im:6795346 ELISA Kit, wu:fa55e01 ELISA Kit, wu:fb02e11 ELISA Kit, wu:fc29a09 ELISA Kit, wu:fi27h08 ELISA Kit, TNF receptor superfamily member 21 ELISA Kit, tumor necrosis factor receptor superfamily member 21 ELISA Kit, tumor necrosis factor receptor superfamily, member 21 ELISA Kit, TNFRSF21 ELISA Kit, tnfrsf21 ELISA Kit, Tnfrsf21 ELISA Kit
- 基因ID
- 27242
- UniProt
- O75509
- 途径
- Regulation of Lipid Metabolism by PPARalpha
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