电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

MMP7 ELISA 试剂盒

MMP7 适用: 人 Colorimetric Sandwich ELISA 0.15-100 ng/mL Cell Culture Supernatant
产品编号 ABIN625058
发货至: 中国
  • 抗原 See all MMP7 ELISA试剂盒
    MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))
    适用
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.15-100 ng/mL
    最低检测浓度
    0.15 ng/mL
    应用范围
    ELISA
    原理
    Human MMP-7 ELISA Kit for Cell Culture Supernatants samples.
    样品类型
    Cell Culture Supernatant
    Analytical Method
    Quantitative
    特异性
    This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, 9, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
    灵敏度
    150 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling MMP7 ELISA Kit
    Top Product
    Discover our top product MMP7 ELISA Kit
  • 应用备注
    Recommended Dilution for serum and plasma samples10 - 100 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • Garratt, Sutanto, Ling, Looi, Iosifidis, Martinovich, Shaw, Kicic-Starcevich, Knight, Ranganathan, Stick, Kicic: "Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis." in: The European respiratory journal, Vol. 46, Issue 2, pp. 384-94, (2015) (PubMed).

    Erbel, Tyka, Helmes, Akhavanpoor, Rupp, Domschke, Linden, Wolf, Doesch, Lasitschka, Katus, Gleissner: "CXCL4-induced plaque macrophages can be specifically identified by co-expression of MMP7+S100A8+ in vitro and in vivo." in: Innate immunity, Vol. 21, Issue 3, pp. 255-65, (2015) (PubMed).

    Manjari, Jyothy, Kumar, Prabhakar, Nallari, Venkateshwari: "Association of matrix metalloproteinase-7 (-181A/G) promoter polymorphism in chronic pancreatitis." in: The Indian journal of medical research, Vol. 140, Issue 5, pp. 609-15, (2015) (PubMed).

    Zhang, Shively: "Acceleration of bone repair in NOD/SCID mice by human monoosteophils, novel LL-37-activated monocytes." in: PLoS ONE, Vol. 8, Issue 7, pp. e67649, (2013) (PubMed).

    Hickman, Sargent: "The application of the use of radioactive phosphorus (32P) in larvae: a new approach for identification of adult insect sources." in: Investigative radiology, Vol. 1, Issue 2, pp. 104-5 (PubMed).

  • 抗原 See all MMP7 ELISA试剂盒
    MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))
    别名
    MMP-7 (MMP7 产品)
    别名
    MAT ELISA Kit, MPMM ELISA Kit, MMP-7 ELISA Kit, MPSL1 ELISA Kit, PUMP-1 ELISA Kit, matrilysin ELISA Kit, LOC727698 ELISA Kit, MMP7 ELISA Kit, Matrilysin ELISA Kit, matrix metallopeptidase 7 ELISA Kit, MMP7 ELISA Kit, Mmp7 ELISA Kit
    背景
    Gene Names: MMP7 MPSL1 PUMP1
    Protein names: Matrilysin (EC 3.4.24.23) (Matrin) (Matrix metalloproteinase-7) (MMP-7) (Pump-1 protease) (Uterine metalloproteinase)
    基因ID
    4316
    UniProt
    P09237
    途径
    Production of Molecular Mediator of Immune Response
You are here:
客服