MMP7 ELISA 试剂盒
-
- 抗原 See all MMP7 ELISA试剂盒
- MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.15-100 ng/mL
- 最低检测浓度
- 0.15 ng/mL
- 应用范围
- ELISA
- 原理
- Human MMP-7 ELISA Kit for Cell Culture Supernatants samples.
- 样品类型
- Cell Culture Supernatant
- Analytical Method
- Quantitative
- 特异性
- This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, 9, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- 灵敏度
- 150 pg/mL
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling MMP7 ELISA Kit
- Top Product
- Discover our top product MMP7 ELISA Kit
-
-
- 应用备注
- Recommended Dilution for serum and plasma samples10 - 100 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
-
Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis." in: The European respiratory journal, Vol. 46, Issue 2, pp. 384-94, (2015) (PubMed).
: "CXCL4-induced plaque macrophages can be specifically identified by co-expression of MMP7+S100A8+ in vitro and in vivo." in: Innate immunity, Vol. 21, Issue 3, pp. 255-65, (2015) (PubMed).
: "Association of matrix metalloproteinase-7 (-181A/G) promoter polymorphism in chronic pancreatitis." in: The Indian journal of medical research, Vol. 140, Issue 5, pp. 609-15, (2015) (PubMed).
: "Acceleration of bone repair in NOD/SCID mice by human monoosteophils, novel LL-37-activated monocytes." in: PLoS ONE, Vol. 8, Issue 7, pp. e67649, (2013) (PubMed).
: "The application of the use of radioactive phosphorus (32P) in larvae: a new approach for identification of adult insect sources." in: Investigative radiology, Vol. 1, Issue 2, pp. 104-5 (PubMed).
: "
-
Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis." in: The European respiratory journal, Vol. 46, Issue 2, pp. 384-94, (2015) (PubMed).
-
- 抗原 See all MMP7 ELISA试剂盒
- MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))
- 别名
- MMP-7 (MMP7 产品)
- 别名
- MAT ELISA Kit, MPMM ELISA Kit, MMP-7 ELISA Kit, MPSL1 ELISA Kit, PUMP-1 ELISA Kit, matrilysin ELISA Kit, LOC727698 ELISA Kit, MMP7 ELISA Kit, Matrilysin ELISA Kit, matrix metallopeptidase 7 ELISA Kit, MMP7 ELISA Kit, Mmp7 ELISA Kit
- 背景
-
Gene Names: MMP7 MPSL1 PUMP1
Protein names: Matrilysin (EC 3.4.24.23) (Matrin) (Matrix metalloproteinase-7) (MMP-7) (Pump-1 protease) (Uterine metalloproteinase)
- 基因ID
- 4316
- UniProt
- P09237
- 途径
- Production of Molecular Mediator of Immune Response
-