Reactive Oxygen Species (ROS) Fluorometric Assay Kit
BCA
Fluorometric
Cell Culture Cells, Tissue Samples
Quantitative
产品编号 ABIN5708944
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Reactive Oxygen Species (ROS) Fluorometric Assay Kit (ABIN5708944)
抗原
Reactive Oxygen Species (ROS)
检测方法
Fluorometric
应用范围
Biochemical Assay (BCA)
样品类型
Cell Culture Cells, Tissue Samples
Analytical Method
Quantitative
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原理
- This kit can be used for detection of ROS content in cells and tissue samples.
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产品特性
- DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.
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试剂未包括
- Fluorescence Microplate reader, Fluorescence Spectrophotometry, Flow Cytometry
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实验时间
- 1 h
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限制
- 仅限研究用
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注意事项
- Protect from light.
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储存条件
- 4 °C,-20 °C
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储存方法
- This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
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有效期
- 12 months
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- Reactive Oxygen Species (ROS)
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别名
- Reactive Oxygen Species
抗原
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