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VEGFR2/CD309 ELISA 试剂盒

Name: VEGFR2/CD309 ELISA 试剂盒
SKU: ABIN5691027
Availability: OutOfStock

VEGFR2 适用: 人 pTyr996 Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate

HUVEC cells were treated with Pervanadate (PV).

ELISA

HUVEC cells were untreated or treated with PV.

ELISA

3 images

产品编号 ABIN5691027

发货至: 中国

Datasheet as PDF

抗原 See all VEGFR2/CD309 (VEGFR2) ELISA试剂盒

VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

抗原表位

pTyr996
适用
13

8

7

2

2

2

2

1

1

1

1

1

1
人

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

原理

Human Phospho-VEGFR2 (Tyr996) ELISA Kit. This assay semi-quantitatively measures VEGFR2 phosphorylated at Tyrosine-996 in cell lysate samples.

样品类型

Cell Samples, Tissue Lysate

Analytical Method

Semi-Quantitative

特异性

This ELISA kit recognizes Human VEGFR2 phosphorylated at site Tyrosine-996.

产品特性
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
试剂未包括
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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板类型

Pre-coated

实验流程
1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

4 °C

储存方法

Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

有效期

6 months
抗原 See all VEGFR2/CD309 (VEGFR2) ELISA试剂盒

VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

别名

VEGFR2 (VEGFR2 产品)

别名

CD309 ELISA Kit, FLK1 ELISA Kit, VEGFR ELISA Kit, VEGFR2 ELISA Kit, 6130401C07 ELISA Kit, Flk-1 ELISA Kit, Flk1 ELISA Kit, Krd-1 ELISA Kit, Ly73 ELISA Kit, VEGFR-2 ELISA Kit, sVEGFR-2 ELISA Kit, Vegfr-2 ELISA Kit, FLK-1 ELISA Kit, flk-1 ELISA Kit, vegfr-2 ELISA Kit, KDR ELISA Kit, FLK/KDR ELISA Kit, flk1 ELISA Kit, flk1b ELISA Kit, kdrb ELISA Kit, si:busm1-205d10.1 ELISA Kit, si:ch211-254j6.1 ELISA Kit, si:ch211-278f21.4 ELISA Kit, wu:fc31a09 ELISA Kit, fk52c05 ELISA Kit, flk ELISA Kit, kdr ELISA Kit, kdra ELISA Kit, vegfr2 ELISA Kit, vegfr4 ELISA Kit, vegr2 ELISA Kit, wu:fk52c05 ELISA Kit, kinase insert domain receptor ELISA Kit, kinase insert domain protein receptor ELISA Kit, vascular endothelial growth factor receptor kdr-like ELISA Kit, kinase insert domain receptor (a type III receptor tyrosine kinase) ELISA Kit, kinase insert domain receptor like ELISA Kit, KDR ELISA Kit, Kdr ELISA Kit, LOC100136647 ELISA Kit, kdr ELISA Kit, kdrl ELISA Kit

基因ID

3791

UniProt

P35968

途径

RTK signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling