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Inhibin alpha ELISA 试剂盒

INHA 适用: 人 Colorimetric Sandwich ELISA 9.9 pg/mL - 1188 pg/mL Plasma, Serum
产品编号 ABIN5680708
发货至: 中国
  • 抗原 See all Inhibin alpha (INHA) ELISA试剂盒
    Inhibin alpha (INHA) (Inhibin, alpha (INHA))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    9.9 pg/mL - 1188 pg/mL
    最低检测浓度
    9.9 pg/mL
    应用范围
    ELISA
    原理
    The Inhibin A enzyme linked immunosorbent assay (ELISA) kit provides materials for the quantitative measurement of dimeric inhibin A in human serum and plasma. It is intended for in vitro diagnostic use as an aid in the diagnosis and monitoring of various hormonal reproductive disorders.
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    灵敏度
    5.45 pg/mL
    组件
    • Inhibin A Calibrators A (Lyophilized)
    • Inhibin A Calibrators B thru F (Lyophilized)
    • Inhibin A Controls I & II (Lyophilized)
    • Inhibin A Coated Microtitration Strips
    • Inhibin A Assay Buffer A
    • Inhibin A Assay Buffer B
    • Inhibin A Biotin Conjugate Concentrate
    • Inhibin A Biotin Conjugate Diluent
    • Inhibin A Streptavidin-Enzyme Conjugate-Ready-to-Use (RTU)
    • TMB Chromogen Solution
    • Stopping Solution
    • Wash Concentrate A
    试剂未包括
    1. Microplate reader capable of absorbance measurement at 450 nm, 405 nm and 630 nm.
    2. Microplate orbital shaker.
    3. Microplate washer.
    4. Semi-automated/manual precision pipette to deliver 10-250 μL.
    5. Vortex mixer.
    6. Deionized water.
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    Discover our top product INHA ELISA Kit
  • 样本量
    50 μL
    实验时间
    4 h
    板类型
    Pre-coated
    试剂准备
    1. Inhibin A Calibrator A: Tap and reconstitute Inhibin A Calibrator A with 1.0 mL deionized water. Solubilize for 10 minutes, mix well and use after reconstitution.
    2. Inhibin A Calibrators B-F and Inhibin A Controls I & II: Tap and reconstitute Inhibin A Calibrators B-F and Inhibin A Controls I & II each with 0.5 mL deionized water. Solubilize for 10 minutes, mix well and use after reconstitution.
    3. Wash Solution: Dilute wash concentrate 25-fold with deionized water. The wash solution is stable for one month at room temperature (23 ± 2 °C) when stored in a tightly sealed bottle.
    4. Microtitration Wells: Select the number of coated wells required for the assay. The remaining unused wells should be placed in the resealable pouch with a desiccant. The pouch must be resealed to protect from moisture.
    5. Inhibin A Antibody-Biotin Conjugate Solution: The Inhibin A Antibody-Biotin Conjugate Concentrate should be diluted at a ratio of 1 part conjugate to 50 parts of Inhibin A Conjugate Diluent, according to the number of wells used. If an entire plate is to be used pipet exactly 220 μL of the Concentrate in to 11 mL of the diluent.
    6. Inhibin A Assay Buffers Premix Solution: The Inhibin A Assay Buffer A (ASB-123A) and Inhibin A Assay Buffer B (ASB-123B) should be mixed by gentle inversion in equal volumes (1:1 ratio) according to the number of wells used. If an entire plate is to be used mix exactly 3 mL of the ASB-123 B in to 3 mL of the ASB-123 A. The pre- solution after 4 hours.
    样品收集
    • Serum is the recommended sample type.
    • Sample handling, processing, and storage requirements depend on the brand of blood collection tube that you use. Please reference the manufacturer's instructions for guidance. Each laboratory should determine the acceptability of its own blood collection tubes and serum separation products.
    • Samples may be stored at 4 °C if assayed within 24 hours, otherwise samples must be stored at -20 °C or -80 °C to avoid loss of bioactivity and contamination.
    • Avoid assaying lipemic, hemolyzed or icteric samples.
    • Avoid repeated freezing and thawing of samples. Thaw samples no more than 3 times.
    • For shipping, place specimens in leak proof containers in biohazard specimen bags with appropriate specimen identification and test requisition information in the outside pocket of the biohazard specimen bag. Follow DOT and IATA requirements when shipping specimens.
    实验流程

    Allow all specimens and reagents to reach room temperature (23 ± 2 °C) and mix thoroughly by gentle inversion before use. Calibrators, controls, and unknowns should be assayed in duplicate. NOTE: All serum samples reading higher than the highest calibrator should be mixed and diluted in the 0 pg/mL reconstituted Calibrator A prior to assay.

    1. Reconstitute Inhibin A Calibrator A with 1.0 mL deionized water.
    2. Reconstitute Inhibin A Calibrators B-F and Inhibin A Controls I & II each with 0.5 mL deionized water. Solubilize for 10 minutes, Mix well.
    3. Label the microtitration strips to be used.
    4. Pipette 50 μL of the Calibrator, Controls and Unknowns to the appropriate wells.
    5. Add 50 μL of Inhibin A Assay Buffers Premix Solution (ASB-123A and ASB- 123B in 1:1 ratio as described under the Preparation of the Reagents section of this package insert) to each well using a repeater pipette.
    6. Incubate the plate, shaking at a fast speed (600-800 rpm) on an orbital microplate shaker, for 2.5 hour at room temperature (23 ± 2 °C).
    7. During the last 20-30 minutes of incubation, prepare the Inhibin A Antibody-Biotin Conjugate Solution by diluting the Inhibin A Biotin Conjugate Concentrate in Inhibin A Conjugate Diluent as described under the Preparation of the Reagents section of this package insert.
    8. Aspirate and wash each strip 5 times with Washing Solution (350 μL/per well) using an automatic microplate washer.
    9. Add 100 μL of the Antibody-Biotin Conjugate Solution to each well using a repeater pipette.
    10. Incubate the plate, shaking at a fast speed (600-800 rpm) on an orbital microplate shaker, for 1 hour at room temperature (23 ± 2 °C).
    11. Aspirate and wash each strip 5 times with the Wash Solution (350 μL/per well) using an automatic microplate washer.
    12. Add 100 μL of the Streptavidin-Enzyme Conjugate-RTU to each well using a repeater pipette.
    13. Incubate the plate, shaking at a fast speed (600-800 rpm) on an orbital microplate shaker, for 30 minutes at room temperature (23 ± 2 °C).
    14. Aspirate and wash each strip 5 times with the Wash Solution (350 μL/per well) using an automatic microplate washer.
    15. Add 100 μL of the TMB chromogen solution to each well using a repeater pipette. Avoid exposure to direct sunlight.
    16. Incubate the wells, shaking at 600-800 rpm on an orbital microplate shaker, for 10-12 min at room temperature (23 ± 2 °C). NOTE: Visually monitor the color development to optimize the incubation time.
    17. Add 100 μL of the stopping solution to each well using a repeater pipette. Read the absorbance of the solution in the wells within 20 minutes, using a microplate reader set to 450 nm. NOTE: Zero calibrator should be programmed as ""Blank"" while reading the optical density. If instrument has a wavelength correction, set the instrument to dual wavelength measurement at 450 nm with background wavelength correction at 630 nm.

    结果分析

    NOTE: The results in this package insert were calculated by plotting the log optical density (OD) data on the y-axis and log Inhibin A concentration on X- axis using a cubic regression curve-fit. Alternatively, log vs. log quadratic regression curve-fit can be used. Other data reduction methods may give slightly different results.

    1. Optimum results can be obtained at incubation temperature of 23 ± 2 °C.
    2. Calculate the mean OD for each calibrator, Control, or Unknown.
    3. Plot the log of the mean OD readings for each of the Calibrators along the y-axis versus log of the Inhibin A concentrations in pg/mL along the x-axis, using a cubic regression curve-fit.
    4. Determine the Inhibin A concentrations of the Controls and unknowns from the calibration curve by matching their mean OD readings with the corresponding Inhibin A concentrations.
    5. Any sample reading higher than the highest Calibrator should be appropriately diluted with the 0 pg/mL (CAL A) and re-assayed.
    6. Any sample reading lower than the analytical sensitivity should be reported as such.
    7. Multiply the value by a dilution factor, if required.

    实验精密度
    Reproducibility of the Inhibin A assay was determined using three serum pool samples for a total of 21 assays. At concentrations between 31.8 and 344.8ng/mL, the CV's ranged between 5.5 and 6.7%.
    限制
    仅限研究用
  • 注意事项
    The following precautions should be observed: a) Follow good laboratory practice. b) Use personal protective equipment. Wear lab coats and disposable gloves when handling immunoassay materials. c) Handle and dispose of all reagents and material in compliance with applicable regulations. WARNING: Potential Biohazardous Material Handle all reagents and patient samples at a Biosafety Level 2, as recommended for any potentially infectious human material in the Centers for Disease Control/National Institutes of Health manual "Biosafety in Microbiological and Biomedical Laboratories," 5th Edition, 2007. WARNING: Potential Chemical Hazard Some reagents in this kit may contain Pro-Clean 400 and Sodium azide as a preservative. Pro-Clean 400 and Sodium azide in concentrated amounts are irritants to skin and mucous membranes. For further information regarding hazardous substances in the kit, please refer to the MSDS.
    储存条件
    4 °C
  • 抗原 See all Inhibin alpha (INHA) ELISA试剂盒
    Inhibin alpha (INHA) (Inhibin, alpha (INHA))
    别名
    Inhibin A (INHA 产品)
    别名
    si:dkey-91f15.2 ELISA Kit, INHA ELISA Kit, MGC108500 ELISA Kit, AW555078 ELISA Kit, inhibin, alpha ELISA Kit, inhibin alpha subunit ELISA Kit, inhibin alpha ELISA Kit, inhibin alpha L homeolog ELISA Kit, inha ELISA Kit, INHA ELISA Kit, inha.L ELISA Kit, Inha ELISA Kit
    途径
    Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion
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