SRD5A1 ELISA 试剂盒
-
- 抗原 See all SRD5A1 ELISA试剂盒
- SRD5A1 (Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0128 ng/mL
- 组件
-
Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
-
1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
- Discover our best selling SRD5A1 ELISA Kit
- Top Product
- Discover our top product SRD5A1 ELISA Kit
-
Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1) ELISA Kit
SRD5A1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1) ELISA KitSRD5A1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1) ELISA KitSRD5A1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1) ELISA KitSRD5A1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1) ELISA KitSRD5A1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1) ELISA KitSRD5A1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1) ELISA KitSRD5A1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
-
1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
-
- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原 See all SRD5A1 ELISA试剂盒
- SRD5A1 (Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1))
- 别名
- Steroid 5 Alpha Reductase 1 (SRD5a1) (SRD5A1 产品)
- 别名
- zgc:153768 ELISA Kit, 0610031P22Rik ELISA Kit, 4930435F02Rik ELISA Kit, S5AR 1 ELISA Kit, Srd5a-1 ELISA Kit, steroid 5 alpha-reductase 1 ELISA Kit, steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) ELISA Kit, 3-oxo-5-alpha-steroid 4-dehydrogenase 1 ELISA Kit, SRD5A1 ELISA Kit, srd5a1 ELISA Kit, Bm1_06570 ELISA Kit, CC1G_14953 ELISA Kit, Srd5a1 ELISA Kit
- 背景
- Alternative name: 3-Oxo-5 Alpha-Steroid Delta 4-Dehydrogenase Alpha 1
- 基因ID
- 6715
- UniProt
- P18405
- 途径
- Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process
-
