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ADH7 ELISA 试剂盒

ADH7 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
产品编号 ABIN5674862
发货至: 中国
  • 抗原 See all ADH7 ELISA试剂盒
    ADH7 (Alcohol Dehydrogenase 7 (Class IV), mu Or sigma Polypeptide (ADH7))
    适用
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.312 ng/mL - 20 ng/mL
    最低检测浓度
    0.312 ng/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0125 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
    Top Product
    Discover our top product ADH7 ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all ADH7 ELISA试剂盒
    ADH7 (Alcohol Dehydrogenase 7 (Class IV), mu Or sigma Polypeptide (ADH7))
    别名
    Alcohol Dehydrogenase 7 (ADH7) (ADH7 产品)
    别名
    ADH4 ELISA Kit, AI325182 ELISA Kit, Adh-3 ELISA Kit, Adh-3e ELISA Kit, Adh-3t ELISA Kit, Adh3 ELISA Kit, Adh3-e ELISA Kit, Adh3-t ELISA Kit, Adh4 ELISA Kit, Adt-1 ELISA Kit, ADH7 ELISA Kit, adh6 ELISA Kit, adh7 ELISA Kit, adh7.L ELISA Kit, alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide ELISA Kit, uncharacterized LOC100232857 ELISA Kit, alcohol dehydrogenase class 4 mu/sigma chain ELISA Kit, alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide S homeolog ELISA Kit, ADH7 ELISA Kit, Adh7 ELISA Kit, LOC100232857 ELISA Kit, LOC100512795 ELISA Kit, adh7.S ELISA Kit
    背景
    Alternative name: Alcohol dehydrogenase class 4 mu/sigma chain, Alcohol dehydrogenase class IV mu/sigma chain, Gastric alcohol dehydrogenase
    基因ID
    131
    UniProt
    P40394
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