CYP11B2 ELISA 试剂盒
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- 抗原 See all CYP11B2 ELISA试剂盒
- CYP11B2 (Cytochrome P450, Family 11, Subfamily B, Polypeptide 2 (CYP11B2))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0112 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原 See all CYP11B2 ELISA试剂盒
- CYP11B2 (Cytochrome P450, Family 11, Subfamily B, Polypeptide 2 (CYP11B2))
- 别名
- Aldosterone Synthase (ALDOS) (CYP11B2 产品)
- 别名
- ALDOS ELISA Kit, CPN2 ELISA Kit, CYP11B ELISA Kit, CYP11BL ELISA Kit, CYPXIB2 ELISA Kit, P-450C18 ELISA Kit, P450C18 ELISA Kit, P450aldo ELISA Kit, Cpn2 ELISA Kit, Cyp11b ELISA Kit, Cyp11b-2 ELISA Kit, Cp45as ELISA Kit, Cyp11b3 ELISA Kit, RNCP45AS ELISA Kit, cytochrome P450 family 11 subfamily B member 2 ELISA Kit, cytochrome P450, family 11, subfamily b, polypeptide 2 ELISA Kit, aldosterone synthase ELISA Kit, cytochrome P450, family 11, subfamily B, polypeptide 2 ELISA Kit, cytochrome P450, subfamily XIB (steroid 11-beta-hydroxylase), polypeptide 2 ELISA Kit, CYP11B2 ELISA Kit, Cyp11b2 ELISA Kit
- 背景
- Alternative name: CPN2, CYP11B, CYP11BL, CYP11B2, P450C18, P450aldo, Steroid 18-hydroxylase, Aldosterone-synthesizing enzyme, Cytochrome P450 Family 11 Subfamily B Polypeptide 2
- 基因ID
- 231
- UniProt
- P15121
- 途径
- ACE Inhibitor Pathway, Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, C21-Steroid Hormone Metabolic Process, Feeding Behaviour
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