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APEX1 ELISA 试剂盒

APEX1 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
产品编号 ABIN5674748
发货至: 中国
  • 抗原 See all APEX1 ELISA试剂盒
    APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
    适用
    • 5
    • 2
    • 2
    • 1
    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.312 ng/mL - 20 ng/mL
    最低检测浓度
    0.312 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0143 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all APEX1 ELISA试剂盒
    APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
    别名
    APEX Nuclease 1 (APEX1) (APEX1 产品)
    别名
    apex1 ELISA Kit, MGC76280 ELISA Kit, MGC79645 ELISA Kit, MGC78928 ELISA Kit, APE ELISA Kit, APE1 ELISA Kit, APEN ELISA Kit, APEX ELISA Kit, APX ELISA Kit, HAP1 ELISA Kit, REF1 ELISA Kit, Apex ELISA Kit, REF-1 ELISA Kit, BAP1 ELISA Kit, apex1a ELISA Kit, hm:zehn1532 ELISA Kit, wu:fc13d11 ELISA Kit, zgc:66204 ELISA Kit, Ref-1 ELISA Kit, apurinic/apyrimidinic endodeoxyribonuclease 1 ELISA Kit, APEX nuclease (multifunctional DNA repair enzyme) 1 ELISA Kit, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog ELISA Kit, DNA-(apurinic or apyrimidinic site) lyase ELISA Kit, apurinic/apyrimidinic endonuclease 1 ELISA Kit, APEX1 ELISA Kit, apex1 ELISA Kit, apex1.L ELISA Kit, CNG03640 ELISA Kit, CNH01280 ELISA Kit, nth ELISA Kit, Spirs_4119 ELISA Kit, Palpr_0103 ELISA Kit, Apex1 ELISA Kit
    背景
    Alternative name: APE, APE1, APEN, APEX, APX, HAP1, REF1, Multifunctional DNA Repair Enzyme, APEX Nuclease 1, Redox factor-1, DNA-(apurinic or apyrimidinic site) lyase
    基因ID
    79116
    UniProt
    P43138
    途径
    DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
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