TNFRSF1B ELISA 试剂盒
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- 抗原 See all TNFRSF1B ELISA试剂盒
- TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B). No significant cross-reactivity or interference between Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) and analogues was observed.
- 灵敏度
- 0.063 ng/mL
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Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) ELISA Kit
TNFRSF1B 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) ELISA KitTNFRSF1B 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) ELISA KitTNFRSF1B 适用: 人 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum
Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) ELISA KitTNFRSF1B 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) ELISA KitTNFRSF1B 适用: 人 AA 23-257 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine
Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) ELISA KitTNFRSF1B 适用: 小鼠 AA 23-258 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum
Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) ELISA KitTNFRSF1B 适用: 人 Soluble Colorimetric Sandwich ELISA 7.81-500 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all TNFRSF1B ELISA试剂盒
- TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
- 别名
- Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B 产品)
- 别名
- CD120b ELISA Kit, TBPII ELISA Kit, TNF-R-II ELISA Kit, TNF-R75 ELISA Kit, TNFBR ELISA Kit, TNFR1B ELISA Kit, TNFR2 ELISA Kit, TNFR80 ELISA Kit, p75 ELISA Kit, p75TNFR ELISA Kit, TNF-RII/TNFRSF1B ELISA Kit, TNFR-II ELISA Kit, TNFRSF1B ELISA Kit, TNF-R2 ELISA Kit, TNF-alphaR2 ELISA Kit, TNFRII ELISA Kit, TNFalpha-R2 ELISA Kit, Tnfr-1 ELISA Kit, Tnfr2 ELISA Kit, rank ELISA Kit, zgc:163064 ELISA Kit, TNF receptor superfamily member 1B ELISA Kit, tumor necrosis factor receptor superfamily, member 1b ELISA Kit, tumor necrosis factor receptor superfamily, member 1B ELISA Kit, TNFRSF1B ELISA Kit, Tnfrsf1b ELISA Kit, tnfrsf1b ELISA Kit
- 背景
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Gene Name: Tumor Necrosis Factor Receptor Superfamily, Member 1B
Gene Aliases: CD120B, P75, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR2, TNFR80, P75tnfr, Tumor necrosis factor receptor 2, p80 TNF-alpha receptor, Etanercept
- 基因ID
- 7133
- UniProt
- P20333
- 途径
- NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway
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