PKC epsilon CLIA Kit
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- 抗原 See all PKC epsilon (PRKCE) CLIA Kits
- PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56 ng/mL - 100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Protein Kinase C Epsilon (PKCe). No significant cross-reactivity or interference between Protein Kinase C Epsilon (PKCe) and analogues was observed.
- 灵敏度
- 0.07 ng/mL
- Top Product
- Discover our top product PRKCE ELISA Kit
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Epsilon (PKCe). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Protein Kinase C Epsilon (PKCe). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Protein Kinase C Epsilon (PKCe) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase C Epsilon (PKCe) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase C Epsilon (PKCe) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all PKC epsilon (PRKCE) CLIA Kits
- PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))
- 别名
- Protein Kinase C Epsilon (PRKCE 产品)
- 别名
- PKCE CLIA Kit, nPKC-epsilon CLIA Kit, 5830406C15Rik CLIA Kit, PKC[e] CLIA Kit, PKCepsilon CLIA Kit, Pkce CLIA Kit, R75156 CLIA Kit, fj59b07 CLIA Kit, prkce CLIA Kit, wu:fj59b07 CLIA Kit, pkce CLIA Kit, pkc-epsilon CLIA Kit, PRKCH CLIA Kit, protein kinase C epsilon CLIA Kit, protein kinase C, epsilon CLIA Kit, protein kinase C, epsilon a CLIA Kit, PRKCE CLIA Kit, Prkce CLIA Kit, prkcea CLIA Kit, prkce CLIA Kit
- 背景
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Gene Name: Protein Kinase C Epsilon
Gene Aliases: PKC-E, PRKCE, PRKC-E, NPKC-Epsilon
- 基因ID
- 5581
- UniProt
- Q02156
- 途径
- TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling
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