NOX1 CLIA Kit
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- 抗原 See all NOX1 CLIA Kits
- NOX1 (NADPH Oxidase 1 (NOX1))
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适用
- 大鼠
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 312.5 pg/mL - 20000 pg/mL
- 最低检测浓度
- 312.5 pg/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). No significant cross-reactivity or interference between Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) and analogues was observed.
- 灵敏度
- 11.88 pg/mL
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NADPH Oxidase 1 (NOX1) ELISA Kit
NOX1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
NADPH Oxidase 1 (NOX1) ELISA KitNOX1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
NADPH Oxidase 1 (NOX1) ELISA KitNOX1 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
NADPH Oxidase 1 (NOX1) ELISA KitNOX1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
NADPH Oxidase 1 (NOX1) ELISA KitNOX1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
NADPH Oxidase 1 (NOX1) ELISA KitNOX1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all NOX1 CLIA Kits
- NOX1 (NADPH Oxidase 1 (NOX1))
- 别名
- Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1 产品)
- 别名
- GP91-2 CLIA Kit, MOX1 CLIA Kit, NOH-1 CLIA Kit, NOH1 CLIA Kit, NOX1a CLIA Kit, NOX1alpha CLIA Kit, Nox-1 CLIA Kit, Nox1 CLIA Kit, NADPH oxidase 1 CLIA Kit, NOX1 CLIA Kit, Nox1 CLIA Kit, nox1 CLIA Kit
- 背景
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Gene Name: Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1
Gene Aliases: MOX1, GP91-2, NOH1, NADPH Oxidase 1, Mitogenic Oxidase(Pyridine Nucleotide-Dependent Superoxide-Generating, NADH/NADPH mitogenic oxidase subunit P65-MOX
- 基因ID
- 114243
- UniProt
- Q9WV87
- 途径
- Regulation of Systemic Arterial Blood Pressure by Hormones, Proton Transport
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