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C5A CLIA Kit

C5a 适用: 大鼠 Chemiluminescent Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
产品编号 ABIN5653254
发货至: 中国
  • 抗原 See all C5A (C5a) CLIA Kits
    C5A (C5a) (Complement Component 5a (C5a))
    适用
    • 7
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    大鼠
    检测方法
    Chemiluminescent
    实验类型
    Sandwich ELISA
    检测范围
    1.56 ng/mL - 100 ng/mL
    最低检测浓度
    1.56 ng/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Complement Component 5a (C5a). No significant cross-reactivity or interference between Complement Component 5a (C5a) and analogues was observed.
    灵敏度
    0.07 ng/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    2 - 3 h
    板类型
    Pre-coated
    实验流程
    The microplate provided in this kit has been pre-coated with an antibody specific to Complement Component 5a (C5a). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement Component 5a (C5a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement Component 5a (C5a) level in the sample or standard.,
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 5a (C5a) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 5a (C5a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all C5A (C5a) CLIA Kits
    C5A (C5a) (Complement Component 5a (C5a))
    别名
    Complement Component 5a (C5a 产品)
    别名
    C5a CLIA Kit, C5b CLIA Kit, CPAMD4 CLIA Kit, Hc CLIA Kit, RGD1561905 CLIA Kit, complement C5 CLIA Kit, C5 CLIA Kit
    背景

    Gene Name: Complement Component 5a

    UniProt
    P08650
    途径
    Complement System, Carbohydrate Homeostasis
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