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COL4A1 ELISA 试剂盒

COL4A1 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
产品编号 ABIN5653130
发货至: 中国
  • 抗原 See all COL4A1 ELISA试剂盒
    COL4A1 (Collagen, Type IV, alpha 1 (COL4A1))
    适用
    • 1
    • 1
    • 1
    • 1
    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    3.12 ng/mL - 200 ng/mL
    最低检测浓度
    3.12 ng/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Collagen Type IV Alpha 1 (COL4a1). No significant cross-reactivity or interference between Collagen Type IV Alpha 1 (COL4a1) and analogues was observed.
    灵敏度
    1.21 ng/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type IV Alpha 1 (COL4a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type IV Alpha 1 (COL4a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type IV Alpha 1 (COL4a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type IV Alpha 1 (COL4a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type IV Alpha 1 (COL4a1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type IV Alpha 1 (COL4a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all COL4A1 ELISA试剂盒
    COL4A1 (Collagen, Type IV, alpha 1 (COL4A1))
    别名
    Collagen Type IV Alpha 1 (COL4A1 产品)
    别名
    col4a1 ELISA Kit, wu:fc02g11 ELISA Kit, HANAC ELISA Kit, ICH ELISA Kit, POREN1 ELISA Kit, arresten ELISA Kit, Bru ELISA Kit, Col4a-1 ELISA Kit, Del(8)44H ELISA Kit, Raw ELISA Kit, Svc ELISA Kit, collagen, type IV, alpha 5 (Alport syndrome) ELISA Kit, collagen type IV alpha 1 chain ELISA Kit, collagen, type IV, alpha 1 ELISA Kit, col4a5 ELISA Kit, COL4A1 ELISA Kit, Col4a1 ELISA Kit
    背景

    Gene Name: Collagen Type IV Alpha 1

    Gene Aliases: COL4-A1, Arresten, Collagen Alpha-1(IV)chain

    途径
    Skeletal Muscle Fiber Development, Growth Factor Binding
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