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Bcl-2 CLIA Kit

BCL2 适用: 人 Chemiluminescent Sandwich ELISA 4.69 ng/mL - 300 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
产品编号 ABIN5652139
发货至: 中国
  • 抗原 See all Bcl-2 (BCL2) CLIA Kits
    Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
    适用
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    检测方法
    Chemiluminescent
    实验类型
    Sandwich ELISA
    检测范围
    4.69 ng/mL - 300 ng/mL
    最低检测浓度
    4.69 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of B-Cell Leukemia/Lymphoma 2 (Bcl2). No significant cross-reactivity or interference between B-Cell Leukemia/Lymphoma 2 (Bcl2) and analogues was observed.
    灵敏度
    0.17 ng/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    2 - 3 h
    板类型
    Pre-coated
    实验流程
    The microplate provided in this kit has been pre-coated with an antibody specific to B-Cell Leukemia/Lymphoma 2 (Bcl2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to B-Cell Leukemia/Lymphoma 2 (Bcl2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the B-Cell Leukemia/Lymphoma 2 (Bcl2) level in the sample or standard.,
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell Leukemia/Lymphoma 2 (Bcl2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell Leukemia/Lymphoma 2 (Bcl2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all Bcl-2 (BCL2) CLIA Kits
    Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
    别名
    B-Cell Leukemia/Lymphoma 2 (BCL2 产品)
    别名
    Bcl-2 CLIA Kit, PPP1R50 CLIA Kit, bcl-2 CLIA Kit, AW986256 CLIA Kit, C430015F12Rik CLIA Kit, D630044D05Rik CLIA Kit, D830018M01Rik CLIA Kit, BCL-2 CLIA Kit, Bcl2 CLIA Kit, BCL2, apoptosis regulator CLIA Kit, B cell leukemia/lymphoma 2 CLIA Kit, B-cell CLL/lymphoma 2a CLIA Kit, B-cell CLL/lymphoma 2 protein CLIA Kit, BCL2 CLIA Kit, Bcl2 CLIA Kit, bcl2a CLIA Kit, BCL-2 CLIA Kit
    背景

    Gene Name: B-Cell Leukemia/Lymphoma 2

    Gene Aliases: B-Cell CLL/Lymphoma 2

    基因ID
    596
    UniProt
    P10415
    途径
    MAPK Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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