BCL11A ELISA 试剂盒
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- 抗原 See all BCL11A ELISA试剂盒
- BCL11A (B-Cell CLL/lymphoma 11A (Zinc Finger Protein) (BCL11A))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of B-Cell CLL/Lymphoma 11A (Bcl11A). No significant cross-reactivity or interference between B-Cell CLL/Lymphoma 11A (Bcl11A) and analogues was observed.
- 灵敏度
- 0.109 ng/mL
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B-Cell CLL/lymphoma 11A (Zinc Finger Protein) (BCL11A) ELISA Kit
BCL11A 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 11A (Bcl11A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 11A (Bcl11A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 11A (Bcl11A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell CLL/Lymphoma 11A (Bcl11A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 11A (Bcl11A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 11A (Bcl11A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all BCL11A ELISA试剂盒
- BCL11A (B-Cell CLL/lymphoma 11A (Zinc Finger Protein) (BCL11A))
- 别名
- B-Cell CLL/Lymphoma 11A (BCL11A 产品)
- 别名
- 2810047E18Rik ELISA Kit, BCL-11A ELISA Kit, Ctip1 ELISA Kit, D930021L15Rik ELISA Kit, Evi9 ELISA Kit, Evi9a ELISA Kit, Evi9b ELISA Kit, Evi9c ELISA Kit, mKIAA1809 ELISA Kit, BCL11A-L ELISA Kit, BCL11A-S ELISA Kit, BCL11A-XL ELISA Kit, BCL11a-M ELISA Kit, CTIP1 ELISA Kit, EVI9 ELISA Kit, HBFQTL5 ELISA Kit, ZNF856 ELISA Kit, bcl11a ELISA Kit, wu:fi09f10 ELISA Kit, zgc:136898 ELISA Kit, B cell CLL/lymphoma 11A (zinc finger protein) ELISA Kit, B-cell CLL/lymphoma 11A ELISA Kit, B-cell CLL/lymphoma 11A L homeolog ELISA Kit, B-cell CLL/lymphoma 11Aa ELISA Kit, Bcl11a ELISA Kit, BCL11A ELISA Kit, bcl11a.L ELISA Kit, bcl11aa ELISA Kit
- 背景
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Gene Name: B-Cell CLL/Lymphoma 11A
Gene Aliases: BCL11A-L, BCL11A-S, BCL11A-XL, CTIP1, EVI9, Ecotropic Viral Integration Site 9, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 homolog, Zinc finger protein 856
- 基因ID
- 53335
- UniProt
- Q9H165
- 途径
- Regulation of Cell Size
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