MSH alpha ELISA 试剂盒
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- 抗原 See all MSH alpha ELISA试剂盒
- MSH alpha
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 123.46 pg/mL - 10000 pg/mL
- 最低检测浓度
- 123.46 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Alpha-Melanocyte Stimulating Hormone (aMSH). No significant cross-reactivity or interference between Alpha-Melanocyte Stimulating Hormone (aMSH) and analogues was observed.
- 灵敏度
- 48.2 pg/mL
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MSH alpha ELISA Kit
适用: 人 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
MSH alpha ELISA Kit适用: 小鼠 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
MSH alpha ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Plasma, Serum
MSH alpha ELISA Kit适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
MSH alpha ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
MSH alpha ELISA Kit适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
MSH alpha ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
MSH alpha ELISA Kit适用: 兔 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Alpha-Melanocyte Stimulating Hormone (aMSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Alpha-Melanocyte Stimulating Hormone (aMSH) and unlabeled Alpha-Melanocyte Stimulating Hormone (aMSH) (Standards or samples) with the pre-coated antibody specific to Alpha-Melanocyte Stimulating Hormone (aMSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Alpha-Melanocyte Stimulating Hormone (aMSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Alpha-Melanocyte Stimulating Hormone (aMSH) in the sample.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Melanocyte Stimulating Hormone (aMSH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Melanocyte Stimulating Hormone (aMSH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all MSH alpha ELISA试剂盒
- MSH alpha
- 别名
- Alpha-Melanocyte Stimulating Hormone (MSH alpha 产品)
- 别名
- ACTH ELISA Kit, CLIP ELISA Kit, LPH ELISA Kit, MSH ELISA Kit, NPP ELISA Kit, POC ELISA Kit, AMSH ELISA Kit, BE ELISA Kit, Beta-LPH ELISA Kit, Clip ELISA Kit, Gamma-LPH ELISA Kit, Npp ELISA Kit, Pomc-1 ELISA Kit, Pomc1 ELISA Kit, alpha-MSH ELISA Kit, alphaMSH ELISA Kit, beta-MSH ELISA Kit, gamma-MSH ELISA Kit, Pomc2 ELISA Kit, proopiomelanocortin ELISA Kit, STAM binding protein ELISA Kit, pro-opiomelanocortin-alpha ELISA Kit, POMC ELISA Kit, STAMBP ELISA Kit, Pomc ELISA Kit
- 背景
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Gene Name: Alpha-Melanocyte Stimulating Hormone
Gene Aliases: α-MSH, Intermedins, Alpha-Melanotropin, Alpha-Melanocortin, Alpha-Intermedin
- 途径
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
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