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beta Actin CLIA Kit

ACTB 适用: 人, 大鼠, 小鼠 Chemiluminescent Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
产品编号 ABIN5651364
发货至: 中国
  • 抗原 See all beta Actin (ACTB) CLIA Kits
    beta Actin (ACTB) (Actin, beta (ACTB))
    适用
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    人, 大鼠, 小鼠
    检测方法
    Chemiluminescent
    实验类型
    Sandwich ELISA
    检测范围
    1.56 ng/mL - 100 ng/mL
    最低检测浓度
    1.56 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Actin Beta (ACTb). No significant cross-reactivity or interference between Actin Beta (ACTb) and analogues was observed.
    灵敏度
    0.05 ng/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    2 - 3 h
    板类型
    Pre-coated
    实验流程
    The microplate provided in this kit has been pre-coated with an antibody specific to Actin Beta (ACTb). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Actin Beta (ACTb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Actin Beta (ACTb) level in the sample or standard.,
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actin Beta (ACTb) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actin Beta (ACTb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all beta Actin (ACTB) CLIA Kits
    beta Actin (ACTB) (Actin, beta (ACTB))
    别名
    Actin Beta (ACTB 产品)
    别名
    BRWS1 CLIA Kit, PS1TP5BP1 CLIA Kit, ACTA1 CLIA Kit, Actx CLIA Kit, E430023M04Rik CLIA Kit, beta-actin CLIA Kit, ps1tp5bp1 CLIA Kit, act-2 CLIA Kit, actb CLIA Kit, B-actin CLIA Kit, LOC100174883 CLIA Kit, LOC100304816 CLIA Kit, A CLIA Kit, ACT5C CLIA Kit, Ac5C CLIA Kit, Act CLIA Kit, Act-5C CLIA Kit, Act5 CLIA Kit, Act5c CLIA Kit, Actin CLIA Kit, Actin/BAP47 CLIA Kit, Actin5C CLIA Kit, BAP47 CLIA Kit, Bap47 CLIA Kit, CG4027 CLIA Kit, Dmel\\CG4027 CLIA Kit, M32055 CLIA Kit, T11 CLIA Kit, act CLIA Kit, act 5C CLIA Kit, act5C CLIA Kit, actin CLIA Kit, actin5C CLIA Kit, anon-EST:fe2D2 CLIA Kit, beta-actin/Bap47 CLIA Kit, cyt5C CLIA Kit, l(1)G0009 CLIA Kit, l(1)G0010 CLIA Kit, l(1)G0025 CLIA Kit, l(1)G0079 CLIA Kit, l(1)G0117 CLIA Kit, l(1)G0177 CLIA Kit, l(1)G0245 CLIA Kit, l(1)G0330 CLIA Kit, l(1)G0420 CLIA Kit, l(1)G0486 CLIA Kit, Bact CLIA Kit, ACT-5 CLIA Kit, ACTB CLIA Kit, B-ACTZF CLIA Kit, actba CLIA Kit, bact CLIA Kit, bactin1 CLIA Kit, bactzf CLIA Kit, wu:fd18f05 CLIA Kit, A26C1B CLIA Kit, POTE2alpha CLIA Kit, POTEACTIN CLIA Kit, Beta-actin CLIA Kit, actin beta CLIA Kit, actin, beta CLIA Kit, cytoplasmic actin OlCA1 CLIA Kit, beta-actin CLIA Kit, Actin 5C CLIA Kit, actin gamma 1 CLIA Kit, actin, beta 1 CLIA Kit, actin, beta L homeolog CLIA Kit, beta actin CLIA Kit, POTE ankyrin domain family member F CLIA Kit, actin, beta like 2 CLIA Kit, actin, cytoplasmic 1 CLIA Kit, ACTB CLIA Kit, Actb CLIA Kit, actb CLIA Kit, LOC100174883 CLIA Kit, LOC100304816 CLIA Kit, Act5C CLIA Kit, ACTG1 CLIA Kit, actb1 CLIA Kit, actb.L CLIA Kit, LOC100136352 CLIA Kit, POTEF CLIA Kit, ACTBL2 CLIA Kit, LOC109073280 CLIA Kit
    背景

    Gene Name: Actin Beta

    Gene Aliases: ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed

    途径
    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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