ADAM10 CLIA Kit
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- 抗原 See all ADAM10 CLIA Kits
- ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))
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适用
- 大鼠
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 78.12 pg/mL - 5000 pg/mL
- 最低检测浓度
- 78.12 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 10 (ADAM10). No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 10 (ADAM10) and analogues was observed.
- 灵敏度
- 3.13 pg/mL
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the A Disintegrin And Metalloprotease 10 (ADAM10) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all ADAM10 CLIA Kits
- ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))
- 别名
- A Disintegrin And Metalloprotease 10 (ADAM10 产品)
- 别名
- ADAM10 CLIA Kit, Adam10 CLIA Kit, adam10 CLIA Kit, wu:fc03d12 CLIA Kit, ad10 CLIA Kit, cd156c CLIA Kit, kuz CLIA Kit, kuzbanian CLIA Kit, madm CLIA Kit, xadam10 CLIA Kit, ADAM-10 CLIA Kit, ADAM 10 CLIA Kit, AD10 CLIA Kit, CD156c CLIA Kit, HsT18717 CLIA Kit, MADM CLIA Kit, 1700031C13Rik CLIA Kit, LOC100219653 CLIA Kit, zgc:64203 CLIA Kit, ADAM metallopeptidase domain 10 CLIA Kit, ADAM metallopeptidase domain 10a CLIA Kit, ADAM metallopeptidase domain 10 S homeolog CLIA Kit, a disintegrin and metallopeptidase domain 10 CLIA Kit, ADAM metallopeptidase domain 10b CLIA Kit, ADAM10 CLIA Kit, Adam10 CLIA Kit, adam10a CLIA Kit, adam10.S CLIA Kit, adam10b CLIA Kit
- 背景
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Gene Name: A Disintegrin And Metalloprotease 10
Gene Aliases: CD156-C, CD156c, MADM, Kuz, CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease
- UniProt
- Q10743
- 途径
- Notch Signaling, EGFR Signaling Pathway
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