iST Sample Preparation Kit

ABIN5542716 产品详细信息, 供应商: Log in to see
应用范围
Mass Spectrometry (MS), Sample Preparation (Prep)
选项
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原理 iST Kits offer all-in-one and simple-to-use solutions for reproducible preparation from any starting material to clean peptide mixtures in just 1 hour for LC-MS measurements.
样品类型 Urine, Plasma, Cell Samples, Serum, Tissue Samples
特异性 - single-step lysis, denaturation, alkylation and reduction
- dual clean-up to remove hydrophilic & hydrophobic contaminants
- compatible with standard lab equipment
- results in ready-to-measure peptides
- works with less than 20 μg protein input
- ultrafast sample preparation workflow (total time <2.5 hours)
- more total & exclusive peptide and protein identifications compared to alternative workflows
产品特性 All-in-one for any starting material
- Simple-to-use, sensitive and fast
- Reproducible, reliable and robust
- Only standard lab equipment needed
组件 The kit contains all you need to perform a complete sample preparation. It includes all chemicals to denature, reduce and alkylate proteins as well as the enzymes to perform a tryptic digest and a final clean up.
试剂未包括 Common lab equipment is required for the sample preparation.
应用备注 - proteome discovery
- validation of biosimilars
- immunoprecipitations
- body fluid analysis (blood, CSF, serum, urine, and more)
说明

Sample preparation is one of the essential steps of bottom-up proteomics. The sample preparation kit is designed to assist you achieving best results with few sample preparation steps and little hands-on time.

实验时间 4 - 5 h
实验精密度 high reproducibility (R2 >0.9)
限制 仅限研究用
储存条件 RT,-20 °C
厂商提供的图像
 image for iST Sample Preparation Kit (ABIN5542716) iST Sample Preparation Kit
有引用在: Karg, Smets, Ryan, Forné, Qin, Mulholland, Kalideris, Imhof, Bultmann, Leonhardt: "Ubiquitome Analysis Reveals PCNA-Associated Factor 15 (PAF15) as a Specific Ubiquitination Target of UHRF1 in Embryonic Stem Cells." in: Journal of molecular biology, 2017 (PubMed).