RFP-Trap®

ABIN509416 产品详细信息, 供应商: Log in to see
抗原
  • RFP
  • RNF76
  • tripartite motif containing 27
  • TRIM27
适用
Discosoma
6
1
1
宿主
Camelid (Camelidae)
抗体类型
Recombinant Antibody
应用范围
Affinity Measurement (AM), Chromatin Immunoprecipitation (ChIP), Enzyme Activity Assay (EAA), Immunoprecipitation (IP), Mass Spectrometry (MS), Protein Complex Immunoprecipitation (Co-IP), Pull-Down Assay (Pull-Down), Purification (Purif)
选项
Supplier
Log in to see
Supplier Product No.
Log in to see
原理 For biochemical analyses of RFP fusion proteins.
品牌 RFP-Trap®
样品类型 Cell Extracts
交叉反应 (详细) RFP-Trap efficiently pulls down various Red fluorescent proteins derived from DsRed, e.g. mRFP1, mCherry, mOrange, mPlum but also mRuby, mKate2 and RFP-tagged fusion proteins. No cross-reaction to DsRed, mRFPruby, TagRFP and all GFPs can be detected.
产品特性 Antibodies - extremely powerful tools in biomedical research - are large complex molecules (~ 150 kDa) consisting of two heavy and two light chains. Due to their complex structure, the use of antibodies is often limited and hindered by batch-to-batch variations.

Camelidae (camels, dromedaries, llamas and alpacas) possess functional antibodies devoid of light chains, so-called heavy chain antibodies (hcAbs). hcAbs recognize and bind their antigens via a single variable domain (VHH). These VHH domains are the smallest intact antigen binding fragments (~ 13 kDa).

Nano-Traps are based on single domain antibody fragments (VHHs) derived from alpaca.
纯化方法 Purified protein
组件 RFP-Trap® uncoupled
试剂未包括 Lysis buffer (CoIP), 10x RIPA buffer, Dilution buffer, Wash buffer, Elution buffer
别名 RFP (RFP ELISA Kit 摘要)
应用备注 Red fluorescent proteins (RFP) and variants thereof are widely used to study protein localization and dynamics. For biochemical analyses including mass spectroscopy and enzyme activity measurements these RFP-fusion proteins and their interacting factors can be isolated fast and efficiently (one step) via immunoprecipitation using the RFP-Trap. The RFPTrap_A enables purification of any protein of interest fused to RFP.
实验时间 1.5 h
实验流程
  • Robust and versatile tool for biochemical analyses of RFP-fusion proteins
  • Short incubation times (5 - 30 min)
  • Quantitative isolation of fusion proteins and transiently bound factors from cell extracts or organelles
  • Low unspecifi c binding
  • No contaminating heavy and light chains of conventional antibodies
  • Applicable in Chromatin Immunoprecipitation (ChIP)
限制 仅限研究用
状态 Liquid
浓度 250 µL resin (1mg/mL)
缓冲液 1 x PBS,0.01% Sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Do not freeze.
储存条件 4 °C
有效期 12 months
厂商提供的图像
 image for RFP-Trap® (ABIN509416) RFP-Trap A
有引用在: Hellewell, Gong, Schärich, Christofidou, Adams: "Modulation of the Extracellular Matrix Patterning of Thrombospondins by Actin Dynamics and Thrombospondin Oligomer State." in: Bioscience reports, 2015 (PubMed).

Qin, Wolf, Liu, Link, Smets, Mastra, Forné, Pichler, Hörl, Fellinger, Spada, Bonapace, Imhof, Harz, Leonhardt: "DNA methylation requires a DNMT1 ubiquitin interacting motif (UIM) and histone ubiquitination." in: Cell research, Vol. 25, Issue 8, pp. 911-29, 2015 (PubMed).

Calderón-Noreña, González-Novo, Orellana-Muñoz, Gutiérrez-Escribano, Arnáiz-Pita, Dueñas-Santero, Suárez, Bougnoux, Del Rey, Sherlock, dEnfert, Correa-Bordes, de Aldana: "A single nucleotide polymorphism uncovers a novel function for the transcription factor Ace2 during Candida albicans hyphal development." in: PLoS genetics, Vol. 11, Issue 4, pp. e1005152, 2015 (PubMed).

Platani, Trinkle-Mulcahy, Porter, Jeyaprakash, Earnshaw: "Mio depletion links mTOR regulation to Aurora A and Plk1 activation at mitotic centrosomes." in: The Journal of cell biology, Vol. 210, Issue 1, pp. 45-62, 2015 (PubMed).

Hachez, Veljanovski, Reinhardt, Guillaumot, Vanhee, Chaumont, Batoko: "The Arabidopsis abiotic stress-induced TSPO-related protein reduces cell-surface expression of the aquaporin PIP2;7 through protein-protein interactions and autophagic degradation." in: The Plant cell, Vol. 26, Issue 12, pp. 4974-90, 2015 (PubMed).

Luo, Xin, Du, Ye, Wei: "Dimerization mediated by a divergent FHA domain is essential for the DNA damage and spindle functions of fission yeast Mdb1." in: The Journal of biological chemistry, 2015 (PubMed).

Hobbs, DePianto, Jacob, Han, Chung, Batazzi, Poll, Guo, Han, Ong, Zheng, Taube, Čiháková, Wan, Coulombe: "Keratin-dependent regulation of Aire and gene expression in skin tumor keratinocytes." in: Nature genetics, Vol. 47, Issue 8, pp. 933-8, 2015 (PubMed).

Bauch, Koliwer, Buck, Hönck, Kreienkamp: "Subcellular sorting of the G-protein coupled mouse somatostatin receptor 5 by a network of PDZ-domain containing proteins." in: PLoS ONE, Vol. 9, Issue 2, pp. e88529, 2014 (PubMed).

Hagen, Mattay, Räuber, Müller, Arndt: "Characterization and inhibition of AF10-mediated interaction." in: Journal of peptide science : an official publication of the European Peptide Society, Vol. 20, Issue 6, pp. 385-97, 2014 (PubMed).

Mass, Wachten, Aschenbrenner, Voelzmann, Hoch: "Murine Creld1 controls cardiac development through activation of calcineurin/NFATc1 signaling." in: Developmental cell, Vol. 28, Issue 6, pp. 711-26, 2014 (PubMed).

Janbon, Ormerod, Paulet, Byrnes, Yadav, Chatterjee, Mullapudi, Hon, Billmyre, Brunel, Bahn, Chen, Chen, Chow, Coppée, Floyd-Averette, Gaillardin, Gerik, Goldberg, Gonzalez-Hilarion, Gujja, Hamlin et al.: "Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation. ..." in: PLoS genetics, Vol. 10, Issue 4, pp. e1004261, 2014 (PubMed).

Yepes, Koch, Waldvogel, Garcia-Betancur, Lopez: "Reconstruction of mreB expression in Staphylococcus aureus via a collection of new integrative plasmids." in: Applied and environmental microbiology, Vol. 80, Issue 13, pp. 3868-78, 2014 (PubMed).

Sechi, Colotti, Belloni, Mattei, Frappaolo, Raffa, Fuller, Giansanti: "GOLPH3 is essential for contractile ring formation and Rab11 localization to the cleavage site during cytokinesis in Drosophila melanogaster." in: PLoS genetics, Vol. 10, Issue 5, pp. e1004305, 2014 (PubMed).

Jaiswal, Lauritzen, Scheffer, Sakaguchi, Bunkenborg, Simon, Kallunki, Jäättelä, Nylandsted: "S100A11 is required for efficient plasma membrane repair and survival of invasive cancer cells." in: Nature communications, Vol. 5, pp. 3795, 2014 (PubMed).

Niehl, Pasquier, Ferriol, Mély, Heinlein: "Comparison of the Oilseed rape mosaic virus and Tobacco mosaic virus movement proteins (MP) reveals common and dissimilar MP functions for tobamovirus spread." in: Virology, Vol. 456-457, pp. 43-54, 2014 (PubMed).

Stagi, Klein, Gould, Bewersdorf, Strittmatter: "Lysosome size, motility and stress response regulated by fronto-temporal dementia modifier TMEM106B." in: Molecular and cellular neurosciences, Vol. 61, pp. 226-40, 2014 (PubMed).

Köster, Meyer, Weinholdt, Smith, Lummer, Speth, Grosse, Weigel, Staiger: "Regulation of pri-miRNA processing by the hnRNP-like protein AtGRP7 in Arabidopsis." in: Nucleic acids research, Vol. 42, Issue 15, pp. 9925-36, 2014 (PubMed).

Robles Luna, Peña, Borniego, Heinlein, Garcia: "Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein." in: Virology, Vol. 441, Issue 2, pp. 152-61, 2013 (PubMed).

Ono, Yamada, Endo, Avolio, Lamond: "Analysis of human protein replacement stable cell lines established using snoMEN-PR vector." in: PLoS ONE, Vol. 8, Issue 4, pp. e62305, 2013 (PubMed).

Montesinos, Langhans, Sturm, Hillmer, Aniento, Robinson, Marcote: "Putative p24 complexes in Arabidopsis contain members of the delta and beta subfamilies and cycle in the early secretory pathway." in: Journal of experimental botany, Vol. 64, Issue 11, pp. 3147-67, 2013 (PubMed).

您还需要查找其他产品吗?