Prolactin CLIA Kit
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- 抗原 See all Prolactin (PRL) CLIA Kits
- Prolactin (PRL)
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Immunoenzymometric assay: The essential reagents required for an immunoenzymometric assay include high affinity and specificity antibodies (enzyme labelled and immobilized), with different and distinct epitope recognition, in excess, and native antigen. In this procedure, the immobilization takes place during the assay at the surface of a microplate well through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal anti-PRL antibody. Upon mixing monoclonal biotinylated antibody, the enzyme-labeled antibody and a serum containing the native antigen, reaction results between the native antigen and the antibodies, without competition or steric hindrance, to form a soluble sandwich complex.
- Analytical Method
- Quantitative
- 产品特性
- The Quantitative Determination of Prolactin Hormone Concentration in Human Serum by a Microplate Chemiluminescense Assay (CLIA)
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- Discover our best selling PRL ELISA Kit
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- Discover our top product PRL ELISA Kit
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Prolactin (PRL) ELISA Kit
PRL 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: Cow Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum
Prolactin (PRL) ELISA KitPRL 适用: Cow Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: 犬 Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: 绵羊 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: 山羊 Colorimetric Competition ELISA 31.25 IU/mL - 2000 IU/mL Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: 小鸡 Colorimetric Competition ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Prolactin (PRL) ELISA KitPRL 适用: 兔 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- All products that contain human serum have been found to be non-reactive for Hepatitis B Surface Antigen, HIV 1&2 and HCV Antibodies by FDA required tests. Since no known test can offer complete assurance that infectious agents are absent, all human serum products should be handled as potentially hazardous and capable of transmitting disease. Good laboratory procedures for handling blood products can be found in the Center for Disease Control / National Institute of Health, "Biosafety in Microbiological and Biomedical Laboratories," 2nd Edition, 1988, HHS Publication No. (CDC) 88-8395.
- 样本量
- 25 μL
- 板类型
- Pre-coated
- 实验流程
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Specimien Collection and Preparation:
The specimens shall be blood, serum in type and the usual precautions in the collection of venipuncture samples should be observed. For accurate comparison to established normal values, a fasting morning serum sample should be obtained. The blood should be collected in a plain redtop venipuncture tube without additives or anti-coagulants. Allow the blood to clot. Centrifuge the specimen to separate the serum from the cells. Samples may be refrigerated at 2_x001E_8oC for a maximum period of five (5) days. If the specimen(s) cannot be assayed within this time, the sample(s) may be stored at temperatures of _x001E_20oC for up to 30 days. Avoid repetitive freezing and thawing. When assayed in duplicate, 0.100ml of the specimen is required.Reagent Preparation:
1. Wash Buffer Dilute contents of Wash Concentrate to 1000ml with distilled or deionized water in a suitable storage container. Store diluted buffer at room temperature 20-27(C. 2. Working Signal Reagent Solution - Store at 2 - 8(C. Determine the amount of reagent needed and prepare by mixing equal portions of Signal Reagent A and Signal Reagent B in a clean container. For example, add 1 ml of A and 1ml of B per two (2) eight well strips (A slight excess of solution is made). Discard the unused portion if not used within 36 hours after mixing. If complete utilization of the reagents is anticipated, within the above time constraint, pour the contents of Signal Reagent B into Signal Reagent A and label accordingly. _x000E_Test Procedure:
Before proceeding with the assay, bring all reagents, serum references and controls to room temperature (20-27(C). 1. Format the microplate wells for each serum reference, control and patient specimen to be assayed in duplicate. Replace any unused microwell strips back into the aluminum bag, seal and store at 2-8(C 2. Pipette 0.025 ml (25l) of the appropriate serum reference, control or specimen into the assigned well. Add 0.100 ml (100l) of hPRL Tracer Reagent to all wells. 4. Swirl the plate gently for 20-30 seconds to mix and cover. 5. Incubate 45 minutes at room temperature. 6. Discard the contents of the microplate by decantation or aspiration. If decanting, blot the plate dry with absorbent paper. 7. Add 350l of wash buffer (see Reagent Preparation Section), decant (tap and blot) or aspirate. Repeat four (4) additional times for a total of five (5) washes. An automatic or manual plate washer can be used. Follow the manufacturers instruction for proper usage. If a squeeze bottle is employed, fill each well by depressing the container (avoiding air bubbles) to dispense the wash. Decant the wash and repeat four (4) additional times. Add 0.100 ml (100l) of working signal reagent to all wells (see Reagent Preparation Section). Always add reagents in the same order to minimize reaction time differences between wells Incubate at room temperature in the dark for five (5) min. 10. Read the relative light units in each well, for minimum 0.5 1.0 seconds, using a microplate luminometer. The results should be read within thirty (30) minutes of adding the substrate solution. - 限制
- 仅限研究用
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- 抗原 See all Prolactin (PRL) CLIA Kits
- Prolactin (PRL)
- 别名
- Prolactin hormone (PRL) (PRL 产品)
- 别名
- PRL CLIA Kit, prolactin CLIA Kit, PRLB CLIA Kit, PRLSD1 CLIA Kit, Prl1a1 CLIA Kit, Prol CLIA Kit, RATPRLSD1 CLIA Kit, RNPROL CLIA Kit, AV290867 CLIA Kit, prl CLIA Kit, prolactin CLIA Kit, prolactin, gene 2 L homeolog CLIA Kit, PROLACTIN protein CLIA Kit, prolactin-like CLIA Kit, PRL CLIA Kit, prl.2.L CLIA Kit, Prl CLIA Kit, LOC100136792 CLIA Kit, LOC100136580 CLIA Kit, PROLACTIN CLIA Kit, LOC101843376 CLIA Kit
- 背景
- Prolactin hormone (PRL), secreted from the lactotrophs of the anterior pituitary, is a protein consisting of a single polypeptide chain containing approximately 200 amino acids. The primary biological action of the hormone is on the mammary gland where it is involved in the growth of the gland and in the induction and maintenance of milk production. There is evidence to suggest that prolactin may be involved in steroidogenesis in the gonad, acting synergistically with luteinizing hormone (LH). High levels of prolactin appear to inhibit steroidogenesis as well as inhibiting LH and follicle stimulating hormone (FSH) synthesis at the pituitary gland (1, 2). The clinical usefulness of the measurement of prolactin hormone (PRL) in ascertaining the diagnosis of hyperprolactinemia and for the subsequent monitoring the effectiveness of the treatment has been well established (3, 4). In this method, PRL calibrator, patient specimen or control is first added to a streptavidin coated well. Biotinylated monoclonal and enzyme labeled antibodies (directed against distinct and different epitopes of PRL) are added and the reactants mixed. Reaction between the various PRL antibodies and native PRL forms a sandwich complex that binds with the streptavidin coated to the well. After the completion of the required incubation period, the enzyme-prolactin hormone antibody bound conjugate is separated from the unbound enzyme-prolactin hormone conjugate by aspiration or decantation. The activity of the enzyme present on the surface of the well is quantitated by reaction with a suitable substrate to produce light. The employment of several serum references of known prolactin hormone levels permits the construction of a dose response curve of activity and concentration. From comparison to the dose response curve, an unknown specimen's activity can be correlated with prolactin hormone concentration.
- 途径
- JAK/STAT Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus
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