PD-L1 ELISA 试剂盒
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- 抗原 See all PD-L1 ELISA试剂盒
- PD-L1 (CD274 (PD-L1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156-10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 原理
- This immunoassay kit allows for the in vitro quantitative determination of Human PDL1 concentrations in serum, plasma and other biological fluids. This kit is for Research use only. Not for use in diagnostic/therapeutics procedures.
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 灵敏度
- < 94 pg/mL
- 组件
- plate, standard, diluent, antibodies
- 试剂未包括
- pipettes, tubes, reader, incubator
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- 样本量
- 100 μL
- 实验时间
- 3 - 4 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs an antibody specific for Anti-human PD-L1 coated on a 96-well plate. Standards and samples are pipetted into the wells and PD-L1present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PD-L1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PD-L1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- 限制
- 仅限研究用
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- 注意事项
- protect your eyes
- 储存条件
- 4 °C
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- 抗原 See all PD-L1 ELISA试剂盒
- PD-L1 (CD274 (PD-L1))
- 别名
- PD-L1 (PD-L1 产品)
- 途径
- Cancer Immune Checkpoints
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