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PD-L1 ELISA 试剂盒

PD-L1 适用: 人 Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum
产品编号 ABIN5026969
发货至: 中国
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    PD-L1 (CD274 (PD-L1))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.156-10 ng/mL
    最低检测浓度
    0.156 ng/mL
    应用范围
    ELISA
    原理
    This immunoassay kit allows for the in vitro quantitative determination of Human PDL1 concentrations in serum, plasma and other biological fluids. This kit is for Research use only. Not for use in diagnostic/therapeutics procedures.
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    灵敏度
    < 94 pg/mL
    组件
    plate, standard, diluent, antibodies
    试剂未包括
    pipettes, tubes, reader, incubator
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  • 样本量
    100 μL
    实验时间
    3 - 4 h
    板类型
    Pre-coated
    实验流程
    This assay employs an antibody specific for Anti-human PD-L1 coated on a 96-well plate. Standards and samples are pipetted into the wells and PD-L1present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PD-L1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PD-L1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
    限制
    仅限研究用
  • 注意事项
    protect your eyes
    储存条件
    4 °C
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    PD-L1 (CD274 (PD-L1))
    别名
    PD-L1 (PD-L1 产品)
    途径
    Cancer Immune Checkpoints
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