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PD-L1 ELISA 试剂盒

PD-L1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum
产品编号 ABIN5026968
发货至: 中国
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    PD-L1 (CD274 (PD-L1))
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.156-10 ng/mL
    最低检测浓度
    0.156 ng/mL
    应用范围
    ELISA
    原理
    This immunoassay kit allows for the in vitro quantitative determination of Mouse PDL-1 concentrations in serum, plasma and other biological fluids.
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    灵敏度
    < 0.094 ng/mL
    组件
    plate, standard, diluent, antibodies
    试剂未包括
    pipettes, tubes, reader, incubator
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  • 样本量
    100 μL
    实验时间
    3 - 4 h
    板类型
    Pre-coated
    实验流程
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Mouse PDL-1 antibody was pre-coated into 96-well plates. Biotin conjugated anti-MousePDL-1detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PDL-1 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Mouse PDL-1 can be calculated using the standard curve.
    限制
    仅限研究用
  • 注意事项
    protect your eyes
    储存条件
    4 °C
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    PD-L1 (CD274 (PD-L1))
    别名
    PD-L1 (PD-L1 产品)
    别名
    B7-H ELISA Kit, B7H1 ELISA Kit, PD-L1 ELISA Kit, PDCD1L1 ELISA Kit, PDCD1LG1 ELISA Kit, PDL1 ELISA Kit, A530045L16Rik ELISA Kit, B7h1 ELISA Kit, Pdcd1l1 ELISA Kit, Pdcd1lg1 ELISA Kit, Pdl1 ELISA Kit, RGD1566211 ELISA Kit, CD274 molecule ELISA Kit, CD274 antigen ELISA Kit, programmed cell death 1 ligand 2 ELISA Kit, CD274 ELISA Kit, Cd274 ELISA Kit, PDCD1LG2 ELISA Kit
    途径
    Cancer Immune Checkpoints
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