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Bcl-2 ELISA 试剂盒

BCL2 适用: 人 Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum
产品编号 ABIN5026957
发货至: 中国
  • 抗原 See all Bcl-2 (BCL2) ELISA试剂盒
    Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.156-10 ng/mL
    最低检测浓度
    0.156 ng/mL
    应用范围
    ELISA
    原理
    This immunoassay kit allows for the in vitro quantitative determination of Human Bcl-2 concentrations in serum, plasma and other biological fluids.
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    灵敏度
    < 0.094 ng/mL
    组件
    plate, standard, diluent, antibodies
    试剂未包括
    pipettes, tubes, reader, incubator
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  • 样本量
    100 μL
    实验时间
    3 - 4 h
    板类型
    Pre-coated
    实验流程
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human Bcl-2 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human Bcl-2 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Bcl-2 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human Bcl-2 can be calculated using the standard curve.
    限制
    仅限研究用
  • 注意事项
    protect your eyes
    储存条件
    4 °C
  • 抗原 See all Bcl-2 (BCL2) ELISA试剂盒
    Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
    别名
    Bcl -2 (B cell Lymphoma 2) (BCL2 产品)
    别名
    Bcl-2 ELISA Kit, PPP1R50 ELISA Kit, bcl-2 ELISA Kit, AW986256 ELISA Kit, C430015F12Rik ELISA Kit, D630044D05Rik ELISA Kit, D830018M01Rik ELISA Kit, BCL-2 ELISA Kit, Bcl2 ELISA Kit, BCL2, apoptosis regulator ELISA Kit, B cell leukemia/lymphoma 2 ELISA Kit, B-cell CLL/lymphoma 2a ELISA Kit, B-cell CLL/lymphoma 2 protein ELISA Kit, BCL2 ELISA Kit, Bcl2 ELISA Kit, bcl2a ELISA Kit, BCL-2 ELISA Kit
    途径
    MAPK Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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