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S100A9 ELISA 试剂盒

S100A9 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Plasma, Serum
产品编号 ABIN4993665
发货至: 中国
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    S100A9 (S100 Calcium Binding Protein A9 (S100A9))
    适用
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    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.16 ng/mL - 10 ng/mL
    最低检测浓度
    0.16 ng/mL
    应用范围
    ELISA
    原理
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of S100A9 in Rat serum, plasma and other biological fluids
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This kit recognizes natural and recombinantRatS100A9. No significant cross-reactivity or interference between RatS100A9 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between RatS100A9 and all the analogues.
    灵敏度
    0.09 ng/mL
    组件
    Micro ELISA Plate
    Reference Standard
    Concentrated Biotinylated Detection Ab
    Concentrated HRP Conjugate
    Reference Standard & Sample Diluent
    Biotinylated Detection Ab Diluent
    HRP Conjugate Diluent
    Concentrated Wash Buffer (25×)
    Substrate Reagent
    Stop Solutio
    Plate Sealer
    Product Description
    Certificate of Analysis
    试剂未包括
    Microplate reader with 450nm wavelength filter
    High-precision transferpettor, EP tubes and disposable pipette tips
    37℃ Incubator
    Deionized or distilled water
    Absorbent paper
    Loading slot for Wash Buffer
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  • 说明

    Information on standard material:
    The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

    Information on reagents:
    Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

    Information on antibodies:
    The provided antibodies and their host vary in different kits. All antibodies are affinity purified

    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

    样本量
    100 µL
    板类型
    Pre-coated
    实验流程
    This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to S100A9. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for S100A9and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain S100A9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of S100A9.You can calculate the concentration of S100A9in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
    限制
    仅限研究用
  • 注意事项
    All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
    储存条件
    4 °C/-20 °C
    储存方法
    The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
  • 抗原 See all S100A9 ELISA试剂盒
    S100A9 (S100 Calcium Binding Protein A9 (S100A9))
    Abstract
    S100A9 产品
    别名
    60B8AG ELISA Kit, CAGB ELISA Kit, CFAG ELISA Kit, CGLB ELISA Kit, L1AG ELISA Kit, LIAG ELISA Kit, MAC387 ELISA Kit, MIF ELISA Kit, MRP14 ELISA Kit, NIF ELISA Kit, P14 ELISA Kit, Mrp14 ELISA Kit, S100A9 ELISA Kit, MRP-14 ELISA Kit, p14 ELISA Kit, 60B8Ag ELISA Kit, AW546964 ELISA Kit, BEE22 ELISA Kit, Cagb ELISA Kit, GAGB ELISA Kit, L1Ag ELISA Kit, S100 calcium binding protein A9 ELISA Kit, S100 calcium binding protein A9 (calgranulin B) ELISA Kit, S100A9 ELISA Kit, S100a9 ELISA Kit
    途径
    Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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