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NRF2 ELISA 试剂盒

NFE2L2 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Plasma, Serum
产品编号 ABIN4993598
发货至: 中国
  • 抗原 See all NRF2 (NFE2L2) ELISA试剂盒
    NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.16 ng/mL - 10 ng/mL
    最低检测浓度
    0.16 ng/mL
    应用范围
    ELISA
    原理
    The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This kit recognizes natural and recombinantHumanNFE2L2. No significant cross-reactivity or interference between HumanNFE2L2 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between HumanNFE2L2 and all the analogues.
    灵敏度
    0.1 ng/mL
    组件
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Reference Standard & Sample Diluent
    • Biotinylated Detection Antibody (100 x concentrate)
    • HRP Conjugate (100 x concentrate)
    • Biotinylated Detection Antibody Diluent
    • HRP Conjugate Diluent
    • Substrate Reagent
    • Stop Solution
    • Wash Buffer (25 x concentrate)
    • Instruction manual
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  • 说明

    Information on standard material:
    The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

    Information on reagents:
    Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

    Information on antibodies:
    The provided antibodies and their host vary in different kits. All antibodies are affinity purified

    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

    样本量
    100 µL
    板类型
    Pre-coated
    实验流程
    1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
    2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
    3. Aspirate and wash 3 times.
    4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
    5. Aspirate and wash 5 times.
    6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
    7. Add 50 µL Stop Solution. Read at 450 nm immediately.
    8. Calculation of results.
    试剂准备
    1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
    2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
    3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 10 ng/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 10, 5, 2.5, 1.25, 0.63, 0.32, 0.16, 0 ng/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 10 ng/mL working solution to the first tube and mix up to produce a 5 ng/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
    4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
    5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.
    限制
    仅限研究用
  • 注意事项
    All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
    储存条件
    4 °C/-20 °C
    储存方法
    The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
  • 抗原 See all NRF2 (NFE2L2) ELISA试剂盒
    NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
    别名
    Nuclear Factor, Erythroid Derived 2 Like 2 (NFE2L2) (NFE2L2 产品)
    别名
    Nrf2 ELISA Kit, nfe2l2 ELISA Kit, wu:fc15g09 ELISA Kit, wu:fj67e03 ELISA Kit, NF-E2R2 ELISA Kit, NRF2 ELISA Kit, AI194320 ELISA Kit, nuclear factor, erythroid 2-like 2a ELISA Kit, nuclear factor, erythroid 2 like 2 S homeolog ELISA Kit, nuclear factor, erythroid 2 like 2 ELISA Kit, nuclear factor, erythroid derived 2, like 2 ELISA Kit, nuclear factor, erythroid 2-like 2 ELISA Kit, nfe2l2a ELISA Kit, nfe2l2.S ELISA Kit, NFE2L2 ELISA Kit, Nfe2l2 ELISA Kit
    途径
    ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling
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