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ESM1 ELISA 试剂盒

ESM1 适用: 大鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Plasma, Serum
产品编号 ABIN4993433
发货至: 中国
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    ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))
    适用
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    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    78.13 pg/mL - 5000 pg/mL
    最低检测浓度
    78.13 pg/mL
    应用范围
    ELISA
    原理
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ESM1 in Rat serum, plasma and other biological fluids
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This kit recognizes natural and recombinantRatESM1. No significant cross-reactivity or interference between RatESM1 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between RatESM1 and all the analogues.
    灵敏度
    46.88 pg/mL
    组件
    Micro ELISA Plate
    Reference Standard
    Concentrated Biotinylated Detection Ab
    Concentrated HRP Conjugate
    Reference Standard & Sample Diluent
    Biotinylated Detection Ab Diluent
    HRP Conjugate Diluent
    Concentrated Wash Buffer (25×)
    Substrate Reagent
    Stop Solutio
    Plate Sealer
    Product Description
    Certificate of Analysis
    试剂未包括
    Microplate reader with 450nm wavelength filter
    High-precision transferpettor, EP tubes and disposable pipette tips
    37℃ Incubator
    Deionized or distilled water
    Absorbent paper
    Loading slot for Wash Buffer
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  • 说明

    Information on standard material:
    The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

    Information on reagents:
    Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

    Information on antibodies:
    The provided antibodies and their host vary in different kits. All antibodies are affinity purified

    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

    样本量
    100 µL
    板类型
    Pre-coated
    实验流程
    This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ESM1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ESM1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ESM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ESM1.You can calculate the concentration of ESM1in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
    限制
    仅限研究用
  • 注意事项
    All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
    储存条件
    4 °C/-20 °C
    储存方法
    The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
  • 抗原 See all ESM1 ELISA试剂盒
    ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))
    别名
    Endothelial Cell Specific Molecule 1 (ESM1) (ESM1 产品)
    别名
    ESM1 ELISA Kit, zgc:153027 ELISA Kit, 0610042H23Rik ELISA Kit, AV004503 ELISA Kit, ESM-1 ELISA Kit, Pg25 ELISA Kit, endocan ELISA Kit, endothelial cell specific molecule 1 ELISA Kit, endothelial cell-specific molecule 1 ELISA Kit, ESM1 ELISA Kit, esm1 ELISA Kit, Esm1 ELISA Kit
    途径
    Growth Factor Binding
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